响应面法优化新疆若羌大枣总黄酮提取工艺及抗氧化活性

摘要

This study was undertaken to optimize condition for the extraction of total lfavonoids from Xinjiang Ruoqiang jujubes. One-factor-at-a-time method was used to investigate the effects of ethanol concentration, temperature, extraction time and solid-to-solvent ratio on the extraction yield of total lfavonoids. Maximum extraction yield of total lfavonoids was reached after 90 min of extraction. Using a three-variable, three-level Box-Behnken experimental design coupled with response surface methodology, ethanol concentration, temperature and solid-to-solvent ratio were optimized to be 45%, 90℃and 1:35 (g/mL), respectively. Under the optimized conditions, the actual extraction yield of total lfavonoids was 14.34 mg kaempferol equivalent/g, which was 97.9% as compared to the predicted value (14.647 mg kaempferol equivalent/g). The obtained extract had an ethyl acetate fraction that revealed potent antioxidant activity and was comparable to BHT.%在单因素试验基础上，以乙醇体积分数、提取温度、料液比为自变量，进行三因素三水平Box-Behnken试验设计，利用响应面分析法优化新疆若羌大枣总黄酮提取条件；将粗提物进一步分级萃取并对其抗氧化部位进行考察。结合实际可操作性得出大枣中总黄酮较优提取工艺为乙醇体积分数45%、提取温度90℃、料液比1：35(g/mL)、提取时间90min，总黄酮提取量为14.34mg山奈酚/g，达到预测值(14.647mg山奈酚/g)的97.9%。乙酸乙酯萃取物具有显著的抗氧化活性，与抗氧化剂BHT活性相当。