不同反胶束体系萃取大豆蛋白研究

摘要

利用 AOT/异辛烷/KCl 溶液，SDS/异辛烷-正辛醇/KCL 溶液，CTAB/异辛烷-正辛醇/KCL 溶液三种反胶束体系前萃取大豆蛋白质，针对影响大豆蛋白萃取率的各种因素如 W0、缓冲溶液 pH、萃取温度、萃取时间、离子强度进行了研究，得到了三种反胶束前萃取的最佳工艺：W0均取16；缓冲溶液 pH 分别为6.5、6.5、10；萃取温度分别为45、45、35℃；萃取时间均取30 min；离子强度均取0.05 mol/L。试验结果表明 AOT 和 SDS 反胶束最佳前萃取条件相同，CTAB 反胶束最佳前萃条件中 pH 和萃取温度与以上两种体系不同，其它条件相同。%Sulphosuccinic acid bis(2-ethylhexyl)estersodium salt(AOT),isooctane and KCl solution reverse micelle system,sodium dodecyl sulfate(SDS)/isooctane /n-octyl alcohol and KCl solution reverse micelle system and cetyltrimethyl ammonium bromide(CTAB)/isooctane-heptylane capryl alcohol/KCl buffer solution revese micelle system were used to backward extract soy protein. The effects of W0,pH,temperature,time,ionic strength were analyzed and the optimal conditions were obtained:W 0=16;pH=6.5、6.5、10;temperature=45 ℃、45 ℃、35 ℃ ;time=30 min;ionic strength=0.05 mol/L. The experimental results showed that the highest forward extraction conditions of SDS and AOT reverse micelle were the same,the pH and temperature of CTAB highest forward extraction conditions were different from the above two systems.