AOT/异辛烷反胶束体系W0调控及萃取大豆蛋白研究

摘要

该文对影响丁二酸二异辛酯磺酸钠（AOT）反胶束体系W0的各因素进行了研究，结果表明：随着加水量的增大，反胶束体系W0增大；而离子强度与AOT浓度增大，反胶束体系W0反而减小。采用AOT反胶束体系萃取大豆蛋白质，可得到最佳前萃取工艺为：缓冲溶液pH 6.5、W015、萃取温度45℃、离子强度0.05 mol/L。%The properties of the reverse micelles formed by AOT,isooctane and water was studied. The result showed with the amount of water increases,the W0 value increases;but with the ionic strength increases,the W0 value decreases;with the AOT concentration increases,the volume of water which can be solubilized in the system increases. Sulphosuccinic acid bis(2–ethylhexyl)estersodium salt(AOT),isooctane and KCl solution reverse micelle system was used to backward extract soy protein,the experimental results lead to the conclusion that the highest forward extraction efficiency of soybean protein was reached at the pH 6.5,W0 value 15,temperature 45 ℃,KCl concentration 0.05 mol/L.