clone

摘要： Silvicultural practices applied in managed forest plantations may help counteract the effects of climate change by influencing soil surface CO_(2)efflux(Fs).Understanding the effects of silvicultural practices on Fs will provide unbiased estimates of carbon fluxes and allow better silvicultural decisions for carbon sequestration.Therefore,we assessed how Fs differed seasonally across silvicultural practices(i.e.,stocking levels,clone,fertilization and weed control treatments)and evaluated the effects of soil temperature(Ts)and soil volumetric water content(θv)on Fs across these practices for a mid-rotation(14 year-old)Pinus radiata plantation in the Canterbury region of New Zealand.There were significant differences in Fs(p<0.05)over the four seasons,three levels of stocking,and five clones.The effects of fertilization and weed control applied 12 years previously on Fs were insignificant.Annual estimate of Fs(mean±1 standard deviation)from the study site was 22.7±7.1 t ha^(-1)a^(-1)in the form of CO_(2)(6.2±2.1 t ha^(-1)a^(-1)in the form of C).Fs values were consistently higher in plots with 1250 stems ha^(-1)compared to 2500 stems ha^(-1),which may be related to a strong soil resource limitation because of the close spacing in the latter plantation.Significant differences in Fs across clones suggest that variations in carbon partitioning might explain their growth performance.Silvicultural treatments influenced Fs response to soil temperature(p<0.05),resulting in models explaining 28-49%of the total variance in Fs.These findings provide insights into how silvicultural management decisions may impact Fs in mid-rotation radiata pine plantations,contributing towards developing more precise and unbiased plantation carbon budgets.

摘要： 在海德汉数控系统里,数据存储介质上的宝贵数据无可替代,也绝不允许丢失。例如,维修保养机床前,需要备份数据。或者,定期备份数据确保安全。海德汉Clone为此而生,保护您的数据。海德汉Clone,能快捷的创建数控系统的完整镜像,并随时还原恢复。只需简单的几步操作就能完成备份和还原。海德汉Clone也是一个备份系统,为不同机床的数控系统提供充足的数据存储空间。也可将备份文件发送到计算机或中央服务器中,并可随时从计算机或中央服务器还原。

摘要： The sweet taste of Stevia leaves makes it a potential substitute for table sugar which can be used to sweeten foods and beverages. However, the limited planting materials become a constrained to large production;hence the experiment aims to investigate the different part and methods of propagation for stevia specifically use of different rooting hormones. The experiment was laid out in 3 × 4 factorial arranged in Randomized Complete Block Design. It consists of 3 types of cutting (shoot tips, intermediate stem and basal stem part) and four kinds of commercial hormones (miracle gro, rootech gel, NAA and control). Results showed that the highest percentage survival of stevia was obtained from shoot tips (93.92%) which differed statistically from those intermediate (91.00%) and basal stem cuttings (85.51%). On the other hand, basal stem cutting significantly has the lowest percent survival. Results revealed that shoot tip cuttings treated with Rootech Gel developed roots early (6.92 days), with most number of roots (13.70), longer roots (3.33 cm), and 96.38% survival.

摘要： At present, the research about flower color of Rosa rugosa is a very inno-vative and practical study. Glycosylation modification fulfills an important role in increasing the stability and solubility of anthocyanin in plants. In this study, based on the transcriptional database of R. rugosa, a gene with full length cDNA of 1422bp, encoding 473 amino acids, designated as RrGT2, were isolated from flowers of R. rugosa ‘Zizhi’ and then functionally characterized. According to online software prediction, the molecular formula of the protein encoded by the RrGT2 gene is C2334H3628N602O711S18, the relative molecular mass is 52,075.17 Da, and the theoretical isoelectric point is pI = 4.76. The result of the RrGT2 protein 3D model construction showed that it had the highest homology with the UDP-glycosyltransferase 74F2 protein model in the database (39.53%). Sequence alignments with the NCBI database showed that the RrGT2 protein is a member of the GTB superfamily. Homology analysis revealed that the coding regions of RrGT2 was highly specific among different species, but still had typical conserved amino acid residues called PSPG that are crucial for RrGT2 enzyme activity. RrGT2 transcripts were detected in five flowering stages and seven tissues of R. rugosa ‘Zizhi’, R. rugosa ‘Fenzizhi’ and R. rugosa ‘Baizizhi’, and their expression patterns corresponded with the accumulation of antho-cyanins. Therefore, we speculated that glycosylation of RrGT2 plays a crucial role in anthocyanin biosynthesis in R. rugosa.

摘要： Glycosylation modification fulfills an important role in increasing the stability and solubility of anthocyanin in plants. In this study, based on the transcriptional database of R. rugosa, a gene with full length cDNA of 1161 bp, encoding 386 amino acids, designated as RrGT1, was isolated from flowers of R. rugosa ‘Zizhi’ and then functionally characterized. According to online software prediction, the molecular formula of the protein encoded by the RrGT1 gene is C1879H2964N494O556S14, the relative molecular mass is 41,820.02 Da, and the theoretical isoelectric point is pI = 5.03. The result of the RrGT1 protein 3D model construction showed that it had the highest homology with the UDP-glucose: anthocyanidin 3-O-glucosyltransferase protein model in the database (47.01%). Sequence alignments with the NCBI database showed that the RrGT1 protein is a member of the GTB superfamily. Homology analysis revealed that the coding regions of RrGT1 was highly specific among different species, but still had typical conserved amino acid residues called PSPG that are crucial for RrGT1 enzyme activity. RrGT1 transcripts were detected in five flowering stages and seven tissues of R. rugosa ‘Zizhi’, R. rugosa ‘Fenzizhi’ and R. rugosa ‘Baizizhi’, and their expression patterns corresponded with the accumulation of anthocyanins. Therefore, we speculated that glycosylation of RrGT1 plays a crucial role in anthocyanin biosynthesis in R. rugosa.

摘要： In order to determine if the TFL1 is related with the continuous flowering phenotype of wild Rosa rugosa from Muping, the full-length cDNA sequence of TFL1 Gene was cloned for the first time from the flower buds of wild Rosa rugosafrom Muping with RT-PCR and RACE methods and named as RrTFL1. The full-length cDNA is 973 bp with an open reading frame of 519 bp, encoding 172 amino acids. The derived protein has a molecular weight of 19.48 kD, a calculated pI of 9.13, a c100227 conserved domain at position 1-172, and belongs to PEBP family. The derived protein is a Hydrophilic protein secreted into the cytoplasmic. There is no transmembrane domain and no signal peptide cleavage site, five Ser phosphorylation sites, seven Thr phosphorylation sites, three Tyr phosphorylation sites, one O-glycosylation site, and no N-glycosylation sites. There are 24.42% α-helixes, 36.63% random coil, 27.91% extended peptide chain, and 11.05% β-corner structure. This protein and the TFL1 protein from Rosaceae plants, including Rosa chinensis, share a sequence homology of 87% - 96%. All of the proteins contain a c100227 conserved domain, two highly conserved modules D-P-D-x-P, G-x-H-R, and two functional sites His, Asp. Furthermore, their phylogenetic relationships are consistent with their traditional classifications. These results not only laid a foundation for further researching the expression and function of RrTFL1, but also cultivating new varieties of R. rugosawhich can flower continuously by gene engineering.

摘要： This article proposes the high-speed and high-accuracy code clone detection method based on the combination of tree-based and token-based methods. Existence of duplicated program codes, called code clone, is one of the main factors that reduces the quality and maintainability of software. If one code fragment contains faults (bugs) and they are copied and modified to other locations, it is necessary to correct all of them. But it is not easy to find all code clones in large and complex software. Much research efforts have been done for code clone detection. There are mainly two methods for code clone detection. One is token-based and the other is tree-based method. Token-based method is fast and requires less resources. However it cannot detect all kinds of code clones. Tree-based method can detect all kinds of code clones, but it is slow and requires much computing resources. In this paper combination of these two methods was proposed to improve the efficiency and accuracy of detecting code clones. Firstly some candidates of code clones will be extracted by token-based method that is fast and lightweight. Then selected candidates will be checked more precisely by using tree-based method that can find all kinds of code clones. The prototype system was developed. This system accepts source code and tokenizes it in the first step. Then token-based method is applied to this token sequence to find candidates of code clones. After extracting several candidates, selected source codes will be converted into abstract syntax tree (AST) for applying tree-based method. Some sample source codes were used to evaluate the proposed method. This evaluation proved the improvement of efficiency and precision of code clones detecting.

摘要： In order to reveal the phenomenon of R. rugose pollination incompatibility, the full-length cDNA sequence of S Locus F-box Gene was cloned for the first time from the pollen of Rosa rugose “Zilong wochi” with RT-PCR and RACE methods and named as RrSLF. The full-length cDNA is 1236 bp with an open reading frame of 1122 bp, encoding 343 amino acids. The derived protein has a molecular weight of 43.7 kD, a calculated pI of 6.24, an F-box conserved domain at position 343 - 741, and belongs to F-box family. The derived protein is a Hydrophobicity protein secreted into the cytoplasm. There is no transmembrane domain and no signal peptide cleavage site, twenty-one Ser phosphorylation sites, seven Thr phosphorylation sites, seven Tyr phosphorylation sites, two N-glycosylation sites, and no O-glycosylation sites. There are 22.25% α-helixes, 31.37% random coil, 32.17% extended peptide chain, and 14.21% β-corner structure. This protein and the SFB/SLF protein from Rosaceae Prunus fruit, including Prunus speciose, share a sequence homology of 59% - 61%;all of the proteins contain an F-box conserved domain, two hypervariable regions HVa, HVb, and two variable regions V1, V2. Furthermore, their phylogenetic relationships are consistent with their traditional classifications. These results were meaningful to reveal the molecular mechanism of Rosa rugose pollination incompatibility and improve the theory and techniques of breeding ornamental Rosa rugose.

摘要： 根据《兽药管理条例》和《兽药注册办法》规定,经审查,批准中国兽医药品监察所、北京中海生物科技有限公司、新疆天康畜牧生物技术股份有限公司、新疆畜牧科学院兽医研究所(新疆畜牧科学院动物临床医学研究中心)4家单位申报的小反刍兽疫活疫苗(Clone 9株)

摘要： Objective: To clone cytochrome P450 from Aedes aegypti(Ae. aegypti) and determine the characteristics using bioinformatics tools. Methods: Cytochrome P450 of Ae. aegypti was amplified using polymerase chain reaction, cloned and sequenced. Evolutionary relationship of the sequence was inferred and bioinformatics tools were used to predict subcellular localisation, signal peptide, transmembrane helix, phosphorylation, O-glycosylation, secondary and tertiary structures of the deduced protein. Results: Polymerase chain reaction rather amplified a cytochrome P450 pseudogene which was named CYP4H44P(Gen Bank accession number KF779932). The pseudogene has 1537 nucleotides and an open reading frame of 335 amino acids containing cytochrome P450 motifs except the Wxxx R motif. It is highly homologous to CYP4H28 and CYP4H28v2. Phylogenetic analysis and evolutionary divergence showed strong clustering with CYP4H28 alleles and least divergence from the alleles respectively. The deduced protein was predicted to be found in the cytoplasm and likely to be phosphorylated but devoid of signal peptide, transmembrane helix and O-glycosylated sites. The secondary and tertiary structures were also generated. Conclusions: A cytochrome P450 pseudogene, CYP4H44 P was cloned from Ae. aegypti. The pseudogene is homologous with CYP4H28 alleles and seems to have recently diverged from this group. Isolating this pseudogene is an important step for evaluating its biological role in the mosquito and for the evolutionary analysis of Ae. aegypti CYPs.

摘要： 本发明涉及生物技术领域，具体为一种人胚肾上皮细胞293T‑Clone3单克隆细胞株及其应用。本发明的单克隆细胞株细胞株保藏号为：CCTCC NO：C2019262，具有高的转染效率，转染效率高于商品化的AAV293，转染效率均达到90％以上，每个150mm皿包装的AAV的上清与细胞的总病毒基因拷贝数均在1011以上，最高可达1012，上清中的病毒基因拷贝数均在1011以上，而AAV293包装出来的病毒基因拷贝数在109‑1011。相较于AAV293，本发明的人胚肾上皮细胞293T‑Clone3单克隆细胞株更能满足实验需求。

摘要： 本发明涉及生物技术领域，具体为一种人胚肾上皮细胞293T‑Clone3单克隆细胞株及其应用。本发明的单克隆细胞株细胞株保藏号为：CCTCC NO：C2019262，具有高的转染效率，转染效率高于商品化的AAV293，转染效率均达到90％以上，每个150mm皿包装的AAV的上清与细胞的总病毒基因拷贝数均在10

摘要： 本实用新型公开了一种支持Clone，Erase，数据存储的多功能转接器，包括下盖、按键、PCB板、导光板和上盖，此转接器设计有三种不同的模式，分别为移动硬盘盒模式，CLONE模式，和磁盘擦除Erase模式，磁盘擦除模式又分随机擦除，全0擦除模式和安全擦除模式三小种，功能强大，实用性较强，在支持2.5寸硬盘的同时又能完美支持3.5寸硬盘，可以很方便的连接各种硬盘进行数据存储操作，共享文件，实现读取更方便，快捷，适合存储大容量文件，可以实现更大的存储空间拓展，不再为存储大量的数据文件而担心。

摘要： 本发明提供了一种用Git原生Clone命令克隆指定Commit的方法，包括以下步骤：(1)当用户通过用户原生Git客户端向Git原生服务器发送普通Git命令时，首先用户通过用户原生Git客户端向Git原生服务器发送普通Git clone命令或者其他git命令；(2)用户命令拦截器拦截用户命令，若发现用户命令为普通git命令，则不做任何额外处理，并将用户命令发送给Git原生服务器；(3)Git原生服务器接收用户命令，并将返回信息按照原路径返回至用户原生Git客户端；本发明提供一种可以克隆Git指定Commit的方法，大幅提升在只需要单commit场景下的clone(克隆)效率。