A method for determining the proportion of a fusion protein into a mixture of fusion protein and proteolytic degradation product

摘要

the method for determining the proportion of a fusionsproteins in a mixture of fusion protein and proteolytischem metabolite is the mixture of fusion protein and metabolite with rinderserumalbumin diluted.a part of the diluted mixture is mixed with amylase solution, 30 minutes for walks, et, - amylase reagent is fed and the residual activity of the amylase solution spektrophotometrisch measured. the other part of the diluted mixture with - amylase reagent mixed after mixing amylase solution and added to the enzymkinetik (reaktionsverlauf) spektrophotometrisch measured.all values are in standards, fusion protein, tendamistat, metabolite and ungehemmter amylase reaction and compared by the comparison of the levels of fusion protein in the mixture.