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首页> 外文期刊>农业科学学报：英文版 >Immunogenicity and protective efficacy of DHBV DNA vaccines expressing envelope and capsid fusion proteins in ducks delivered by attenuated Salmonella typhimurium
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Immunogenicity and protective efficacy of DHBV DNA vaccines expressing envelope and capsid fusion proteins in ducks delivered by attenuated Salmonella typhimurium

机译：表达减毒鼠伤寒沙门氏菌的鸭子中表达包膜和衣壳融合蛋白的DHBV DNA疫苗的免疫原性和保护功效

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摘要

Duck hepatitis B virus （DHBV） shares many basic characteristics with hepatitis B virus （HBV） and is an attractive model forvaccine development. In this study, DHBV DNA vaccines were designed to express envelope and capsid fusion proteinsto enhance the breadth of immune response in ducks. Attenuated Salmonella typhimurium （SL7207） was used as a carrierand adjuvant to boost the magnitude of immune response. Based on this strategy, novel DNA vaccines （SL7207-pVAX1-LC and SL7207-pVAX1-SC） were generated. Growth kinetics, genetic stabilities and relative transcription levels of the L,S and C genes introduced by these vaccine strains were measured before inoculation to guarantee safety and efficacy.The relative transcript levels of the CD4 and CD8 T genes and the antibody levels （IgY） in ducks receiving the vaccineswere higher than those in single gene delivered groups. Additionally, the copy number of covalently closed circular DNA inhepatocytes after DHBV challenge also provided evidence that our fusion vaccines could enhance the protective efficiencyagainst DHBV infection in ducks.
机译：鸭乙型肝炎病毒（DHBV）与乙型肝炎病毒（HBV）具有许多基本特征，是疫苗发展的诱人模型。在这项研究中，DHBV DNA疫苗被设计为表达包膜和衣壳融合蛋白，以增强鸭子免疫反应的广度。减毒鼠伤寒沙门氏菌（SL7207）被用作载体和佐剂，以增强免疫反应的强度。基于这种策略，产生了新型的DNA疫苗（SL7207-pVAX1-LC和SL7207-pVAX1-SC）。接种前先检测这些疫苗株引入的L，S和C基因的生长动力学，遗传稳定性和相对转录水平，以确保安全性和有效性。CD4和CD8T基因的相对转录水平和抗体水平（IgY）接受疫苗的鸭中比接受单基因递送的人群更高。此外，DHBV攻击后共价闭合的环状DNA肝细胞的拷贝数也提供了证据，表明我们的融合疫苗可以提高对鸭DHBV感染的保护效率。

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  • 来源
    《农业科学学报：英文版》 |2018年第004期|P.928-939|共12页
  • 作者

    LIU Si-yang123; JIA Ren-yong123; LI Qing-qing123; FENG Dai-shen123; SHEN Hao-yue123; YANG Cui123; WANG Ming-shu123; ZHU De-kang123; CHEN Shun123; LIU Ma-feng123; ZHAO Xin-xin123; YIN Zhong-qiong3; JING Bo3; CHENG An-chun123;

  • 作者单位

    [1]Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China;

    [2]Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China;

    [3]Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    [1]Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China;

    [2]Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China;

    [3]Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    [1]Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China;

    [2]Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China;

    [3]Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    [1]Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China;

    [2]Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China;

    [3]Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    [1]Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China;

    [2]Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China;

    [3]Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    [1]Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China;

    [2]Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China;

    [3]Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    [1]Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China;

    [2]Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China;

    [3]Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    [1]Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China;

    [2]Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China;

    [3]Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    [1]Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China;

    [2]Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China;

    [3]Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    [1]Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China;

    [2]Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China;

    [3]Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    [1]Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China;

    [2]Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China;

    [3]Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    [3]Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    [3]Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    [1]Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China;

    [2]Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China;

    [3]Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

  • 收录信息 中国科学引文数据库(CSCD);
  • 原文格式 PDF
  • 正文语种 CHI
  • 中图分类 农业科学;
  • 关键词

    fusion; DNA; genes; attenuated; Salmonella; typhimurium; （SL7207）; hepatitis; B; virus; （HBV）; duck; hepatitis; B; virus（DHBV）; oral; immunization;

    机译：融合;DNA;基因;减毒;沙门氏菌;鼠伤寒;（SL7207）;肝炎;B;病毒;（HBV）;鸭;肝炎;B;病毒（DHBV）;口服;免疫;
  • 入库时间 2022-08-17 13:10:19
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