• 主题
高级搜索  >
历史搜索 清空历史
您现在的位置：首页> 中文期刊>医药卫生>基础医学>中国病毒学

中国病毒学

中国病毒学

北大核心

CSCD

CSTPCD

Virologica Sinica
本刊由中国科学院武汉病毒研究所、中国微生物学会共同主办、科学出版社出版的学术性双月刊，创刊于1986年，原名为《病毒学杂志》(Virologica　Sinica)，季刊。1991年更名为《中国病毒学》，外文刊名不变，2003年改为双月刊，自创刊以来，发表病毒学研究论文1000多篇，发表论文基金率为65%以上。曾三次荣获湖北省优秀期刊奖，被评为中国生物学核心期刊、基础医学类核心期刊和中国科学引文数据库核心期刊。长期被BA（生物学文摘）、CA（化学文摘）和中国生物学文摘、医学文摘、农业学文摘等国内外20余种文摘及检索刊物收录，为国家科技部信息所“万方数据（ChinaInfo）系统”、清华大学“中国学术期刊光盘版”和“中国期刊网”的期刊源。是CSCI　(中国科学引文索引)、中国生物学和医学期刊的核心期刊。影响因子为0.553　(2003年统计数据)
更多
  • 发文量：1806
  • 被引量：324
  • H指数：3
  • 开始收录时间：
  • CNKI综合因子：
  • 维普期刊影响因子：
  • 万方期刊影响因子：0.42
  • 创刊时间：
  • 国内刊号/CN：42-1706/Q
    国际刊号/ISSN：1674-0769
  • 发行周期：双月刊
    邮发代号：
  • 主管单位：中国科学院
    主办单位：中国科学院武汉病毒研究所，中国微生物学会
中国病毒学-联系信息
  • 主编：陈新文
  • 电话：027-87199157
  • 邮箱：info@virosin.org
  • 地址：武汉武昌区小洪山中区44号
  • 邮编：430071
中国病毒学-获奖情况
  • 1990年湖北省期刊印刷质量奖
  • 1994年-1995年度湖北省优秀期刊
  • 湖北省第一届、第二届、第四届优秀期刊奖
  • 收录汇总
  • 刊内文献检索
  • 投稿信息

刊期浏览

相关中文期刊

相关中文会议

相关外文期刊

相关外文会议

最新期刊

热门期刊

中国病毒学 >2022年第6期
搜索

学科

年度

全选（0）
排序：
共4384条结果
  • 摘要：本文报道利用噬菌体zp-2对青枯假单胞杆菌姜瘟菌株定量检测方法的研究。通过对纯菌的测定,结果表明在每毫升10~3菌落形成单位（CFU）的浓度下,纯菌回收率达81％,检测的敏感性较高,在每毫升10~2（CFU）的条件下也能测到活菌。
  • 摘要：本文将BAS用于固相免疫吸附试验,建立LAB-ELISA抗-HBc/IgM试验方法,并与普通ELISA法进行了比较。实验表明:本法敏感性较普通ELISA高2—4倍,检出率高出后者6.8％,且具有较好的重复性。为血清抗-HBc/IgM检测提供了一种新的、切实可行的方法。本试验采用改良过碘酸盐法制备酶标记抗生物素,以含A蛋白葡萄球菌菌体亲和吸附试验制备抗-μ抗体,在稀释液中加入凝聚正常兔IgG以克服类风湿因子的干扰、考核方法的特异性采用了含A蛋白葡萄球菌菌体吸附试验及抗HBc-抑制试验。
  • 摘要：为获得TMV弱毒,在选择轻症纯毒系的基础上,以亚硝酸诱变处理30、40、50、60分钟,得到了30个无症突变株,经室内交叉保护试验,其中DN60-3对TMV的重花叶株系、条斑株系和山东济南的坏疽型都有显著的干扰作用,而对生产影响不大的黄色花叶株系和黄色环斑株系的干扰作用差。田间试验示范结果,对保护地栽培的番茄花叶病（包括卷叶病）的防病增产效果明显。同时发现,栽培技术差,植株长势弱,则保护放果减低。由于弱毒的保护作用属于持异性免疫,因此,在弱毒应用上,对TMV和CMV混发地区的露地番茄无保护作用。随着抗TMV番茄品种的全面推广,弱毒的利用将会失去重要性,目前一些中小城市番茄品种正处于更新时期,弱毒仍可发挥一定作用。
  • 摘要：＜正＞ 应中国教育委员会和武汉大学的邀请,日本生化学会理事、日本国立遗传学研究所分子生物学系主任石浜明教授于今年5月4日至5月23日来华访问。在武汉大学就原核基因转录的分子机制、RNA多聚酶、RNA多聚酶的启动子选择性、转录的调节机制,以及动物病毒的基因组、转录与复制的分子机制等进行了系统讲授并介绍了最新研究成果。在课堂内外与病毒系师生开展了广泛的交流讨论。参观了该系生化研究室并进行了
  • 摘要：＜正＞ 我们怀着极其悲痛的心情,深切悼念中共党员,一级教授,中国科学院学部委员,本刊主编,著名的病毒学家、教育家高尚荫。高尚荫教授因病于1989年4月24日遽然长逝,享年80岁,这是我们病毒学界的重大损失。
  • 摘要：＜正＞乙肝病毒（HBV）引起的乙型肝炎是人们普遍关注的传染病,目前一般采用血清学方法进行临床检测。中国科学院武汉病毒研究所分子病毒室用α32P—dCTP标记克隆化的HBVDNA片段作分子杂交探针,制备检测药盒,研究HBVDNA在人血清、体液和组织中存在的状态。以HBVDNA作为传染性的指标比血清学方法更为直接可靠。该药盒不需特殊试剂和设备,能在一般科研、医疗、卫生和防疫等部门应用。两年
  • 摘要：＜正＞近年来由于分子生物学的兴起使病毒学作为一门独立学科似乎模糊起来了,难怪一位“分子生物学家”教授宣称病毒学已不存在了。事实真是这样吗?分子生物学是在结构生物化学和细胞遗传学两门学科的基础上成长起来的,而这两门学科的充实和发展又来源于病毒学[有关分子生物学究竟代表什么,见作者的“试论分子生物学”（武汉大学自然科学学报1983年第3期）和“再论分子生物学——科学的革命”（病毒学杂志1987年第1期社论）]。
  • 摘要：＜正＞鸡胚及鸡胚成纤维细胞（CEF）培养已广泛地用于病毒学研究及许多种病毒疫苗的生产。鸡胚及CEF培养中的潜在病毒感染可干扰病毒学研究及疫苗质量。禽网状内皮增生病病毒（REV）是在免疫学上不同于禽白血病病毒的一群逆转录病毒,该群病毒的成员可分别在火鸡、鸡、鸭等禽类引起肿瘤、免疫缺陷综合症、生长迟缓等病理变化。在美国的一些流行病学调查表明,将近20％的鸡群有REV感染,虽然由其引起的经济损失还不清楚。在江苏省调查的三个鸡场中,也发现有一个存在REV感染。
  • 摘要：＜正＞ 中国微生物学会全国干扰素会议首届肝炎临床学术讨论会,于1988年12月13日—15日在武汉召开。来自全国12个省市专家63人,收到论文近50篇。全国干扰素肝炎临床讨论会由中国科学院武汉病毒研究所吴章琦付教授主持,全国干抚素会议负责人杜平教授致开幕词。由著名病毒学家、中国科学院学部委员高尚荫教授作有关临床基础问题的讲话。中华传染病学会主任委员团庚善教授作“乙肝病毒治疗进展”的专题报告。杜平教授作“干扰素研究进展”专题报告。这两个系统而又深入的专题报告。对应前干扰素及其联合治疗乙肝临床研究有十分重要的指导和参考价性。
  • 摘要：＜正＞ 中国微生物学会农业微生物专业委员会于1988年10月21—23日在江西省南昌市召开了全国杀虫微生物学术讨论会,来自21个省、市、自治区的高等院校、科研,生产、管理、出版等60个单位的85名代表出席了会议。这次学术讨论会是杀虫微生物战线上两年一度的盛会,大会共收到126篇论文,其中病原细菌57篇、昆虫病毒40篇、虫生真菌18篇、杀虫抗菌素3篇、微孢子虫3篇、线虫3篇、其它2篇,这反映了我国杀虫微生物科研生产的新景象。会议采用大会报告和专业分组讨论相结合的方式。大会报告有苏云金杆菌各变种间δ-内毒素基因的转移、杀虫素进展、球形芽孢杆菌的研究、昆虫组织培
  • 摘要：从地理位置不同,V—C组不同的13株小麦全蚀病菌中分离到直径23—36纳米的病毒。聚丙烯酰胺凝脉电泳表明这些菌株中的病毒dsRNA的组分及分子量不同。泰安株病毒有分子量为3.45,2.95,1.60,1.52,1.30,1.25,1.15,1.00,＜1.00×10~6的9个dsRNA,而浙江、湖北菌株仅有一个组分、分子量为3×10~6。多数病毒衣壳多肽的的分子量为66×10~3—73×10~3。泰安菌株与烟台菌株病毒抗血清除与张家口菌株、青海菌株无反应外与其它菌株病毒均有反应,但在免疫双扩散中抗血清的滴定度不同。
  • 摘要：＜正＞ 前文分别报道了长叶车前花叶病毒上海分离侏（RMVsh）单克隆抗体的制备及根据它们在不同免疫反应中的特性,将它们分为两组,分别识别性质不同的抗原决定簇。本文采用修改的Friguet方法测定了各组内各单克隆抗体之间的增值反应（Additivity Reaction）特性,并分析了它们识别抗原决定簇的特性。村料与方法一、病毒及单克隆抗体长叶车前花叶病毒上海分离株及其单克隆抗体1H2、7H1、10H1、11H2、12H3、17H6、29H1来源、制备和特性见前文报道。二、抗原饱和曲线的测定抗原饱和曲线测定采用间接ELISA办法,抗原浓度为2μg/ml。
  • 摘要：＜正＞ 《对有机体的感情》是一本传记,评述细胞遗传学家麦克林托克（Barbara McClintock）的生平和科研成果,包括基因转座的发现以及30多年中她在学术生活中的坎坷历程;并且紧密地联系科学和科学研究的哲学思想问题。由于涉及的问题对科研工作可能有所启示,本文主要探讨这些问题的意义。一种新的见解,新的概念往往是从客观事物反映到科学家的头脑而产生的。但是概念成为科学体系的组成部分必得到科学家所属的社会（学术社会）所接受,同时社会的集体智慧对新见解的成长又提供了条件。从整体说,科学知识是由个人的创造和社会的确认之间的相互作用而成的,这种关系有时比较复杂但又微妙地配合,有时这种相互的作用又告失败。失败的原因可能是由于个人因素,或者是由于学术的偏见而没有被社会
  • 摘要：＜正＞ 美国加州斯坦福“时代论坛报”（Times Tribune）1987年12月18日报道,美国基因工程公司（Genenteeh Inc.）发现一种能贴附于爱滋病病毒的分子能有效地防止病毒感染健康细胞,这一发现可能对发展一种治疗爱滋病的新方法有重大意义。在当天出版的“科学”杂志上,南三蕃市的研究者报道:实验业已证明,基因工程合成的分子起着有效地诱使爱滋病病毒远离可能易被攻击的免疫系统细胞的作用。“这是十分有效的,它能完全阻止体外（试管内）的感染,”美国基因工程公司的高级研究人员、该报道的作者Daniel Capon博士说,“但我们尚不清楚它对人是否有疗效。”虽然该工作迄今仍局限于实验室,但基因工程公司和其它实验室的科学家认为这种
  • 摘要：＜正＞ 兔细小病毒分类鉴定研究成果鉴定会,于1987年12月22日在武汉举行。会议由中国科学院武汉分院主持,到会专家有全国著名病毒学家、中国科学院学部委员高尚荫教授,病毒学家刘年翠教授等八名病毒学界老前辈,在对研究报告进行严肃人真审议评价后,专家们一致认为:
  • 摘要：＜正＞ 《中国昆虫病毒电子显微镜图谱》一书由中国科学院武汉病毒研究所张立人主编,世界著名的病毒学家、学部委员高尚荫教授审阅,订于1988年年初由科学出版社出版,全国各地新华书店经售。该书是我国第一部昆虫病毒形态结构和昆虫细胞病理等方面的专著。本书以图为主、文字介绍为辅。全书主要阐明我国发现的农、林害虫和经济昆虫各类昆虫病毒的形态、超微结构、病毒侵染、形态发生、核酸蛋白和细胞病理等特征。书中图片均为我国科技工作者根据自己的材料制作的样品,应用电子计算机图像处理系统和现代电子显微镜拍摄而成。全书图片共有十个部分,包括导图、核型多角体病毒、颗粒体病毒、质型多角体病毒、昆虫痘病毒、非包涵体病毒、昆虫病毒核酸与蛋白质、昆虫病毒的形态发生、昆虫的细胞病理和其它等。此外,还根据文献记载介绍了我国已发现的昆虫病毒及其宿主名录。书后附有昆虫病毒名称中外文索引和参考文献。对于昆虫病毒标本的采
  • 摘要：＜正＞ Kronvall氏等报告:溶血性链球菌的ARI株和AW43株分别能牢固地结合血清中的IgG和IgA。我们发现,AW43株能同时将风疹的非特异红血球凝集抑制因子吸收。因此,用上述菌株,依据血抑试验法,探讨了风疹的早期诊断。材料与方法发病1～5天的风疹患者35名,血清66份,无风疹病史者（而且风疹抗体阴性者）血清115份。
  • 摘要：本文对蛋白质的十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶电泳（PAGE）,酶联印迹法（EITB）的条件和所用试剂的改进进行了讨论。EITB的应用已在各种研究和日常临床检测得到证实。文章着重阐明有关印迹的条件、灵敏度、试剂和仪器的使用局限性、样品的处理和加样诸方面的问题。
  • 摘要：Severe fever with thrombocytopenia syndrome virus（SFTSV） is a highly pathogenic tick-borne bunyavirus that causes lethal infectious disease and severe fever with thrombocytopenia syndrome（SFTS） in humans. The molecular mechanisms and host cellular factors required for SFTSV infection remain uncharacterized. Using a genome-wide CRISPR-based screening strategy, we identified a host cellular protein, sorting nexin 11（SNX11） which is involved in the intracellular endosomal trafficking pathway, as an essential cell factor for SFTSV infection. An SNX11-KO HeLa cell line was established, and SFTSV replication was significantly reduced. The glycoproteins of SFTSV were detected and remained in later endosomal compartments but were not detectable in the endoplasmic reticulum（ER） or Golgi apparatus. pH values in the endosomal compartments of the SNX11-KO cells increased compared with the pH of normal HeLa cells, and lysosomal-associated membrane protein 1（LAMP1） expression was significantly elevated in the SNX11-KO cells. Overall,these results indicated that penetration of SFTSV from the endolysosomes into the cytoplasm of host cells was blocked in the cells lacking SNX11. Our study for the first time provides insight into the important role of the SNX11 as an essential host factor in the intracellular trafficking and penetrating process of SFTSV infection via potential regulation of viral protein sorting, membrane fusion, and other endocytic machinery.
  • 摘要：Japanese encephalitis（JE） is a viral encephalitis disease caused by Japanese encephalitis virus（JEV） infection. Uncontrolled inflammatory responses in the central nervous system（CNS） are a hallmark of severe JE. Although the CCR2–CCL2 axis is important for monocytes trafficking during JEV infection, little is known about its role in CNS trafficking of CD8+T cells. Here, we characterized a mouse model of JEV infection, induced via intravenous injection（i.v.） and delineated the chemokines and infiltrating peripheral immune cells in the brains of infected mice. The CNS expression of chemokines, Ccl2, Ccl3, and Ccl5, and their receptors, Ccr2 or Ccr5, was significantly up-regulated after JEV infection and was associated with the degree of JE pathogenesis. Moreover, JEV infection resulted in the migration of a large number of CD8+T cells into the CNS. In the brains of JEV-infected mice, infiltrating CD8+T cells expressed CCR2 and CCR5 and were found to comprise mainly effector T cells（CD44+CD62 L-）. JEV infection dramatically enhanced the expression of programmed death 1（PD-1） on infiltrating CD8+T cells in the brain, as compared to that on peripheral CD8+T cells in the spleen. This effect was more pronounced on infiltrating CCR2+CD8+T cells than on CCR2-CD8+T cells. In conclusion,we identified a new subset of CD8+T cells（PD1+CCR2+CD8+T cells） present in the CNS of mice during acute JEV infection. These CD8+T cells might play a role in JE pathogenesis.
  • 摘要：A plethora of evidence suggests that protein misfolding and aggregation are underlying mechanisms of various neurodegenerative diseases, such as prion diseases and Alzheimer’s disease（AD）. Like prion diseases, AD has been considered as an infectious disease in the past decades as it shows strain specificity and transmission potential. Although it remains elusive how protein aggregation leads to AD, it is becoming clear that cellular prion protein（PrPC ） plays an important role in AD pathogenesis. Here, we briefly reviewed AD pathogenesis and focused on recent progresses how PrPC contributed to AD development. In addition, we proposed a potential mechanism to explain why infectious agents, such as viruses, conduce AD pathogenesis. Microbe infections cause Aβ deposition and upregulation of PrPC , which lead to high affinity binding between Aβ oligomers and PrPC . The interaction between PrPC and Aβ oligomers in turn activates the Fyn signaling cascade, resulting in neuron death in the central nervous system（CNS）. Thus, silencing PrPC expression may turn out be an effective treatment for PrPC dependent AD.
  • 摘要：＜正＞Dear Editor,In 2009, an outbreak of an unknown infectious disease occurred in rural areas of Hubei Province, China, affecting17 persons, five of whom died. It was initially suspected to be human anaplasmosis（Zhang et al. 2008）. However, the laboratory evidence was insufficient to verify the diagnosis
  • 摘要：＜正＞Dear Editor,Porcine epidemic diarrhea virus（PEDV） is the etiologic agent of porcine epidemic diarrhea（PED）, which is an acute, highly contagious, and devastating enteric viral disease in pigs（Lee 2015）. PEDV is a coronavirus that mainly infects and replicates in villous enterocytes of the small intestine in pigs（Li et al. 2016）. PEDV can infect
  • 摘要：Dengue virus（DENV） infection is a worldwide public health threat. To date, the knowledge about the pathogenesis and progression of DENV infection is still limited. Combining global profiling based on proteomic analysis together with functional verification analysis is a powerful strategy to investigate the interplay between the virus and host cells. In the present study, quantitative proteomics has been applied to evaluate host responses（as indicated by altered proteins and modifications） in human cells（using K562 cell line） upon DENV-2 infection, as DENV-2 spreads most widely among all DENV serotypes. Comparative analysis was performed to define differentially expressed proteins in the infected cells compared to the mock-control, and it revealed critical pathogen-induced changes covering a broad spectrum of host cellular compartments and processes. We also discovered more dramatic changes（＞20%, 160 regulated phosphoproteins）in protein phosphorylation compared to protein expression（14%, 321 regulated proteins）. Most of these proteins/phosphoproteins were involved in transcription regulation, RNA splicing and processing, immune system, cellular response to stimulus, and macromolecule biosynthesis. Western blot analysis was also performed to confirm the proteomic data.Potential roles of these altered proteins were discussed. The present study provides valuable large-scale protein-related information for elucidating the functional emphasis of host cell proteins and their post-translational modifications in virus infection, and also provides insight and protein evidence for understanding the general pathogenesis and pathology of DENV.
  • 摘要：Severe fever with thrombocytopenia syndrome phlebovirus（SFTSV） has a wide host range. Not only has it been found in humans, but also in many wild and domesticated animals. The infection of breeding deer on farms is a particularly worrisome public health concern due to the large amount of human contact and the diverse use of deer products, including raw blood. To investigate the prevalence of breeding domesticated deer, we examined the SFTSV infection rate on deer farms in South Korea from 2015 to 2017. Of the 215 collected blood samples, 0.9%（2/215） were found to be positive for viral RNA by PCR, and sequence analysis showed the highest homology with the KADGH human isolate. Both SFTSVspecific recombinant N and Gn protein-based ELISAs revealed that 14.0%（30/215） and 7.9%（17/215） of collected blood specimens were positive for SFTSV antibody. These results demonstrate that the breeding farm deer are exposed to SFTSV and could be a potential infection source for humans through direct contact or consumption of byproducts.
  • 摘要：The phosphatidylserine-specific phospholipase A1（PLA1 A） is an essential host factor in hepatitis C virus（HCV）assembly. In this study, we mapped the E2, NS2 and NS5 A involved in PLA1 A interaction to their lumenal domains and membranous parts, through which they form oligomeric protein complexes to participate in HCV assembly. Multiple regions of PLA1 A were involved in their interaction and complex formation. Furthermore, the results represented structures with PLA1 A and E2 in closer proximity than NS2 and NS5 A, and strongly suggest PLA1 A-E2’s physical interaction in cells. Meanwhile, we mapped the NS5 A sequence which participated in PLA1 A interaction with the C-terminus of domain 1. Interestingly, these amino acids in the sequence are also essential for viral RNA replication. Further experiments revealed that these four proteins interact with each other. Moreover, PLA1 A expression levels were elevated in livers from HCV-infected patients. In conclusion, we exposed the structural determinants of PLA1 A, E2, NS2 and NS5 A proteins which were important for HCV assembly and provided a detailed characterization of PLA1 A in HCV assembly.
  • 摘要：Chikungunya fever is a vector-borne viral disease transmitted to humans by chikungunya virus（CHIKV）-infected mosquitoes. There have been many outbreaks of CHIKV infection worldwide, and the virus poses ongoing risks to global health. To prevent and control CHIKV infection, it is important to improve the current CHIKV diagnostic approaches to allow for the detection of low CHIKV concentrations and to correctly distinguish CHIKV infections from those due to other mosquito-transmitted viruses, including dengue virus（DENV）, Japanese encephalitis virus（JEV）, and Zika virus（ZIKV）. Here, we produced monoclonal antibodies（mAbs） against the CHIKV envelope 2 protein（CHIKV-E2） and compared their sensitivity and specificity with commercially available m Abs using enzyme-linked immunosorbent assays（ELISA）. Two anti-CHIKV-E2 mAbs, 19-1 and 21-1, showed higher binding affinities to CHIKV-E2 protein than the commercial mAbs did. In particular, the 19-1 m Ab had the strongest binding affinity to inactivated CHIKV. Moreover, the 19-1 mAb had very little cross-reactivity with other mosquito-borne viruses, such as ZIKV, JEV, and DENV. These results suggest that the newly produced anti-CHIKV-E2 mAb, 19-1, could be used for CHIKV diagnostic approaches.
  • 摘要：The study was conducted to explore the mechanisms of sex differences in the response to chronic hepatitis B（CHB） in terms of DNA methylation, SNP genotype, and gene expression. Genomic DNA was isolated from peripheral blood mononuclear cells（PBMCs） of CHB patients and healthy controls and evaluated using the Human Methylation 450 K Assay. The DNA methylation level at hg37 chromosome（CHR） X: 7810800 was further validated using pyrosequencing.SNP genotypes, VCX m RNA expression of PBMCs, and plasma VCX protein concentration were further examined using SNaPshot, RT-qPCR, and Western blot, respectively. Results showed that a total of 5529 CpG loci were differentially methylated between male and female CHB patients. DNA methylation level and CC+CT frequency at CHR X: 7810800,VCX mRNA expression of PBMCs, and plasma VCX protein concentration were higher in female than in male CHB patients. The CHR X: 7810800 locus was hypermethylated in CHB patients with CC+CT genotypes in comparison with those with the TT genotype. In cases of CC+CT genotypes, VCX mRNA expression was negatively correlated with the DNA methylation level. CHB patients with higher levels of HBV DNA, AST, and GGT or higher GPRI scores exhibited lower VCX expression. In conclusion, SNPs and DNA methylation at the CHR X: 7810800 locus cooperatively regulate VCX expression in CHB. The upregulated VCX expression in female CHB patients might represent a mechanism of protection from more severe liver dysfunction and extensive fibrosis, as observed in male CHB patients.
  • 摘要：Conventional influenza vaccines need to be designed and manufactured yearly. However, they occasionally provide poor protection owing to antigenic mismatch. Hence, there is an urgent need to develop universal vaccines against influenza virus. Using nucleoprotein（NP） and extracellular domain of matrix protein 2（M2e） genes from the influenza A virus A/Beijing/30/95（H3N2）, we constructed four recombinant vaccinia virus-based influenza vaccines carrying NP fused with one or four copies of M2e genes in different orders. The recombinant vaccinia viruses were used to immunize BALB/C mice. Humoral and cellular responses were measured, and then the immunized mice were challenged with the influenza A virus A/Puerto Rico/8/34（PR8）. NP-specific humoral response was elicited in mice immunized with recombinant vaccinia viruses carrying full-length NP, while robust M2e-specific humoral response was elicited only in the mice immunized with recombinant vaccinia viruses carrying multiple copies of M2e. All recombinant viruses elicited NP-and M2e-specific cellular immune responses in mice. Only immunization with RVJ-4M2eNP induced remarkably higher levels of IL-2 and IL-10 cytokines specific to M2e. Furthermore, RVJ-4M2eNP immunization provided the highest cross-protection in mice challenged with 20 MLD50 of PR8. Therefore, the cross-protection potentially correlates with both NP and M2e-specific humoral and cellular immune responses induced by RVJ-4M2eNP, which expresses a fusion antigen of full-length NP preceded by four M2e repeats. These results suggest that the rational fusion of NP and multiple M2e antigens is critical toward inducing protective immune responses, and the 4M2eNP fusion antigen may be employed to develop a universal influenza vaccine.
  • 摘要：Severe fever with thrombocytopenia syndrome（SFTS） is an emerging hemorrhagic fever disease caused by SFTSV, a newly discovered phlebovirus that is named after the disease. Currently, no effective vaccines or drugs are available for use against SFTSV infection, as our understanding of the viral pathogenesis is limited. Bortezomib（PS-341）, a dipeptideboronic acid analog, is the first clinically approved proteasome inhibitor for use in humans. In this study, the antiviral efficacy of PS-341 against SFTSV infection was tested in human embryonic kidney HEK293 T（293 T） cells. We employed four different assays to analyze the antiviral ability of PS-341 and determined that PS-341 inhibited the proliferation of SFTSV in 293 T cells under various treatment conditions. Although PS-341 did not affect the virus absorption, PS-341 treatment within a non-toxic concentration range resulted in a significant reduction of progeny viral titers in infected cells.Dual-luciferase reporter assays and Western blot analysis revealed that PS-341 could reverse the SFTSV-encoded nonstructural protein（NS） mediated degradation of retinoic acid-inducible gene-1（RIG-I）, thereby antagonizing the inhibitory effect of NSs on interferons and blocking virus replication. In addition, we observed that inhibition of apoptosis promotes virus replication. These results indicate that targeting of cellular interferon pathways and apoptosis during acute infection might serve as the bases of future therapeutics for the treatment of SFTSV infections.

    中国病毒学的期刊信息

    • 曾用名：病毒学杂志
    • 创刊时间：
    • 地区：
    • 语言：中文
    • 热门主题：
    • 学科分类：

    中国病毒学的获奖情况

    • 1990年湖北省期刊印刷质量奖
    • 1994年-1995年度湖北省优秀期刊
    • 湖北省第一届、第二届、第四届优秀期刊奖

    中国病毒学的收录情况

    • 中国科技论文与引文数据库 (2019)
    • 中国科技论文与引文数据库 (2018)
    • 中国科技论文与引文数据库 (2017)
    • 中国科技论文与引文数据库 (2016)
    • 中国科技论文与引文数据库 (2015)
    • 中国科技论文与引文数据库 (2014)
    • 中国科技论文与引文数据库 (2013)
    • 中国科技论文与引文数据库 (2012)
    • 中国科技论文与引文数据库 (2011)
    • 中国科技论文与引文数据库 (2010)
    • 中国科技论文与引文数据库 (2009)
    • 美国《科学引文索引》 (2019)

    中国病毒学的投稿信息

    • 投稿方式：
    • 投稿须知：点击查看
    • 期刊官方网站：点击前往
    • 版面费：
    • 审稿费：元
    • 通讯地址：武汉武昌区小洪山中区44号
    • 邮编：430071
    • 联系电话：027-87199157
    • 邮箱：info@virosin.org
    • 期刊栏目：研究报告;简报;综述
    免费订阅本刊

    客服邮箱：kefu@zhangqiaokeyan.com

    京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有 出版物经营许可证

    • 客服微信

    • 服务号