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首页> 外文期刊>农业科学学报（英文版） >Immunogenicity and protective efficacy of DHBV DNA vaccines expressing envelope and capsid fusion proteins in ducks delivered by attenuated Salmonella typhimurium
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Immunogenicity and protective efficacy of DHBV DNA vaccines expressing envelope and capsid fusion proteins in ducks delivered by attenuated Salmonella typhimurium

机译：表达减毒鼠伤寒沙门氏菌的鸭子中表达包膜和衣壳融合蛋白的DHBV DNA疫苗的免疫原性和保护功效

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摘要

Duck hepatitis B virus (DHBV) shares many basic characteristics with hepatitis B virus (HBV) and is an attractive model for vaccine development. In this study, DHBV DNA vaccines were designed to express envelope and capsid fusion proteins to enhance the breadth of immune response in ducks. Attenuated Salmonella typhimurium(SL7207)was used as a carrier and adjuvant to boost the magnitude of immune response. Based on this strategy, novel DNA vaccines (SL7207-pVAX1-LC and SL7207-pVAX1-SC) were generated. Growth kinetics, genetic stabilities and relative transcription levels of the L, S and C genes introduced by these vaccine strains were measured before inoculation to guarantee safety and efficacy. The relative transcript levels of the CD4 and CD8 T genes and the antibody levels (IgY) in ducks receiving the vaccines were higher than those in single gene delivered groups. Additionally, the copy number of covalently closed circular DNA in hepatocytes after DHBV challenge also provided evidence that our fusion vaccines could enhance the protective efficiency against DHBV infection in ducks.
机译：Duck乙型肝炎病毒（DHBV）与乙型肝炎病毒（HBV）分享许多基本特征，是一种有吸引力的疫苗开发模型。在该研究中，DHBV DNA疫苗设计用于表达封套和衣壳融合蛋白，以增强鸭子的免疫应答的宽度。减毒的沙门氏菌（SL7207）用作载体和佐剂，以提高免疫应答的幅度。基于该策略，产生新型DNA疫苗（SL7207-PVAX1-LC和SL7207-PVAX1-SC）。在接种之前测量通过这些疫苗菌株引入的L，S和C基因的生长动力学，遗传稳定性和相对转录水平，以保证安全性和功效。 CD4和CD8 T基因的相对转录物水平和接受疫苗的鸭子中的抗体水平（IgY）高于单一基因递送的基团的抗体水平。另外，DHBV挑战后肝细胞中共价闭合圆形DNA的拷贝数也提供了证据表明我们的融合疫苗可以提高鸭子中DHBV感染的保护效率。

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  • 来源
    《农业科学学报（英文版）》 |2018年第4期|928-939|共12页
  • 作者

    LIU Si-yang; LIU Ma-feng; ZHAO Xin-xin; YIN Zhong-qiong; JING Bo; CHENG An-chun; JIA Ren-yong; LI Qing-qing; FENG Dai-shen; SHEN Hao-yue; YANG Cui; WANG Ming-shu; ZHU De-kang; CHEN Shun;

  • 作者单位

    Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

  • 收录信息 中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
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  • 入库时间 2022-08-18 00:23:46
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