首页> 外文OA文献 >Novel dipeptidyl peptidase‐IV and angiotensin‐I‐converting enzyme inhibitory peptides released from quinoa protein by in silico proteolysis
【2h】

Novel dipeptidyl peptidase‐IV and angiotensin‐I‐converting enzyme inhibitory peptides released from quinoa protein by in silico proteolysis

机译:在硅蛋白分解中,从藜麦蛋白释放的新型二肽基肽酶-IV和血管紧张素-I转换酶抑制肽

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

Abstract Quinoa protein has been paid more and more attention because of its nutritional properties and beneficial effects. With the development of bioinformatics, bioactive peptide database and computer‐assisted simulation provide an efficient and time‐saving method for the theoretical estimation of potential bioactivities of protein. Therefore, the potential of quinoa protein sequences for releasing bioactive peptides was evaluated using the BIOPEP database, which revealed that quinoa protein, especially globulin, is a potential source of peptides with dipeptidyl peptidase‐IV (DPP‐IV) and angiotensin‐I‐converting enzyme (ACE) inhibitory activities. Three plant proteases, namely papain, ficin, and stem bromelain, were employed for the in silico proteolysis of quinoa protein. Furthermore, four tripeptides (MAF, NMF, HPF, and MCG) were screened as novel promising bioactive peptides by PeptideRanker. The bioactivities of selected peptides were confirmed using chemical synthesis and in vitro assay. The present work suggests that quinoa protein can serve as a good source of bioactive peptides, and in silico approach can provide theoretical assistance for investigation and production of functional peptides.
机译:摘要藜蛋白已经支付,因为它的营养特性和有益效果的越来越多的关注。随着生物信息学的发展,生物活性肽数据库和计算机辅助模拟提供了蛋白的潜在生物活性的理论估算的效率和节省时间的方法。因此,用于释放生物活性肽藜蛋白质序列的潜在使用BIOPEP数据库,它表明,藜蛋白质进行评价,特别是球蛋白,是与二肽基肽IV(DPP-IV)和肽的血管紧张素I转化的潜在来源酶(ACE)抑制活性。三种植物蛋白酶,木瓜蛋白酶,即,无花果蛋白酶和茎菠萝蛋白酶,是采用藜蛋白质在硅片蛋白水解。此外,四三肽(MAF,NMF,HPF,和MCG)筛选由PeptideRanker新颖有前途的生物活性肽。选择的肽的生物活性,使用化学合成和体外测定证实。本研究表明,藜蛋白质可以作为生物活性肽的良好来源,而在硅片的方法可以提供功能性多肽的研究和生产提供理论支持。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号