Characterization of gold kiwifruit pectin from fruit of different maturities and extraction methods

摘要

Studies on gold kiwifruit pectins are limited. In this work, the characterization of pectin isolated from two different stages of maturity of gold kiwifruit, namely early harvested fruit (EHF) and main harvested fruit (MHF) isolated by three methods (acid, water, enzymatic) was carried out. Pectins isolated from MHF were higher in galacturonic acid content (52-59% w/w) and weight-average molecular weights (M_w, 1.7-3.8 × 10~6g/mol) compared with EHF pectins (29-49% w/w and 0.2-1.7 × 10~6g/mol respectively). Enzymatic treatment gave the highest yield but lowest in M_w, viscosity and mechanical spectra for both maturities. The pectin of both maturities was classified as high-methoxyl pectin with the degree of ester-ification ranged from 82% to 90%. Water-extracted MHF pectin molecules had the highest RMS radius (182.7 nm) and M_w (3.75 × 10~6 g/mol). The water extraction method appeared to retain the native state of pectin molecules compared with acid and enzymatic extraction methods based on the M_w and viscosity data.