Detection of Paracoccidioides brasiliensis gp43 Gene in Sputa by Loop‐Mediated Isothermal Amplification Method

摘要

The fungus is the pathogen of paracoccidioidomycosis (PCM), a systemic mycosis prevalent in Latin America. The loop‐mediated isothermal amplification method (LAMP) was used in this study to detect the presence of in sputa samples from patients with chronic PCM, suspected PCM, and a negative control. The target gp43 gene was amplified in less than 4 hr in 11 of 18 sputa samples tested. The LAMPmethod had the advantage of speed and simplicity compared with the classic diagnostic methods such as the histopathological test or biological material culture and did not require sophisticated technical apparatus. It would be an important aid in cases where immediate treatment would mean patient survival, especially in immune‐suppressed patients. J. Clin. Lab. Anal. 23:139–143 2009. © 2009 Wiley‐Liss, Inc.