Early Detection of Leptospirosis in Cattle Urine Samples by Using Loop-Mediated Isothermal Amplification (LAMP) Method

摘要

Leptopsirosis is a zoonotic disease caused by bacteria infection of the genus Leptopsira sp. It is recognized as endemic diseases in tropical areas. In this study, we developed simple Loop-Mediated Isothermal Amplification (LAMP) method in order to use as an early detection of the disease This study used a number of 8 cattle's urine samples from Wijimulyo Sub-District, and 6 samples from Moyudan Sub-District, Kulon Progo District, Yogyakarta Special Province. All samples were treated by using centrifugation and boiling method, then the isolated DNA were applied into thermocycler. The results shows the ability of Loop-Mediated Isothermal Amplification (LAMP) as an early alternative diagnostic method of leptospirosis which is quickly, accurately and economically.