DETECTION OF NEISSERIA MENINGITIDIS BY LOOP MEDIATED BY LOOP MEDIATED ISOTHERMAL AMPLIFICATION ASSAY

摘要

The invention provides a LAMP assay for detection of meningococcal disease, the test comprising at least one nucleic acid primer set capable of detecting Neisseria meningitides in a LAMP based molecular test, the primer set being chosen from the primer sets listed in Table 1 as LAMP SETS 1 to 12 comprising SEQUENCE IDs from ID:1 to ID:69. Each assay consists of a primer set including of one pair of forward (FIP) and reverse (BIP) inner primers, forward (F3) and reverse (B3) outer primers. The assay may also include loop forward (LF) and/or loop back (LB) primers to accelerate the reaction. Neisseria meningitides serotypes A,B,C,Y and W135 can be detected using the assay of the invention.