stx1 stx2 Primer set for high sensitive real-time multiplex loop-mediated isothermal amplification reaction for determining type of shiga toxin genes stx1 and stx2 of Enterohemorrhagic Escherichia coli and method for determining type of shiga toxin genes of Enterohemorrhagic Escherichia coli using the same

摘要

The present invention relates to a primer set for high sensitivity real time multiplex isothermal amplification reaction for discriminating a cigarette toxin genotype of intestinal hemorrhagic Escherichia coli, a composition and a kit for identifying a cigarette toxicity genotype of intestinal hemorrhagic Escherichia coli comprising the primer set, The present invention relates to a method for identifying a cigarette toxin genotype of Escherichia coli. When the isothermal amplification reaction is performed using the primer set according to the present invention, the intestinal hemorrhagic Escherichia coli can be specifically detected with high sensitivity before the bacterial isolation is completed, , The result can be quantified, and the reaction time can be greatly improved, so that the contamination and the occurrence level caused by enterohemorrhagic Escherichia coli can be confirmed quickly and precisely, and the cigar poisoning can be discriminated at the same time. It can be effectively utilized as an early screening system for detecting a causal agent.;