Evaluation of a new point-of-care oral anti-HCV test for screening of hepatitis C virus infection

摘要

Schematic and procedures of the Well anti-HCV assay. Oral sample collection; mixing of oral liquid with diluted buffer solution and storage in collection bottle; addition of oral samples for testing; schematic of the shell and diagram illustrating the working principle of the internal test strip. HCV antibody first binds to the recombinant protein G conjugated with colloidal gold to form a complex. Under the action of chromatography, the complex binds to the recombinant HCV antigen (core + nonstructural [NS]3 + NS4 + NS5), which was immobilized on the detection area (test-line); and results interpretation