HEPATITUS B MUTANT VIRUS STRAIN, NUCLEIC ACID ISOLATED MOLECULE, CODING MUTANT MAIN SURFACE ANTIGEN OF HEPATITUS B VIRUS STRAIN, VECTOR AND VECTOR SYSTEM FOR THE PREPARATION OF POLYPEPTIDE, A METHOD FOR THE PREPARATION OF POLYPEPTIDE (VARIANTS), PURIFIED POLYPEPTIDE (VARIANTS), OLIGONUCLEOTIDE, A METHOD FOR THE PREPARATION OF ANTIBODIES (VARIANTS), ANTIBODY (VARIANTS), A METHOD FOR THE DETERMINATION OF CHEMICALCOMPOUND FOR THE TREATMENT OF INFECTION OF HEPATITIS VIRUS STRAIN, A COMPOSITION, A METHOD FOR SCREENING OF LIQUIDS OF ORGANISM FOR HEPATITIS B VIRUS, VACCINE AGAINST HEPATITIS

摘要

This invention provides an isolated strain of Hepatitis B virus designated Human Hepatitis B Virus Surface Antigen-'S'-133 Oon Strain (Methionine to Threonine) which constituent viral genome is deposited under Accession Nos. P97121501, P97121502 and P97121503 with the European Collection of Cell Culture on l5th December 1997. This invention also provides an isolated nucleic acid encoding a polypeptide which is a mutant major surface antigen of a strain of hepatitis B virus, such polypeptide having an amino acid sequence which differs from the amino acid sequence of a major surface antigen of a wild type hepatitis B virus in that me amino acid at position number 133 of such polypeptide is a threonine rather than a methionine. This invention also provides an isolated nucleic acid which encodes a peptide, wherein the peptide is encoded by a nucleic acid molecule comprising nucleotides 527 through 595 of SEQ. ID. No. 1 and me purified peptide. This invention also provides various methods of using the disclosed isolated nucleic acid and peptides. This invention further provides various uses of the disclosed isolated nucleic acid, polypeptides and peptides, and antibodies.