新疆枸杞多糖的提取及其单糖组成分析

摘要

In this experiment, we use Lycium barbarums as research materials to extract lycium barbarum polysaccharide by ultrasound-assisted. The effect of temperature, ratio of feed liquid, time and ultrasonic power on the yield of polysaccharide is studied, and the monosaccharide composition of LBP is analyzed by GPC-LLS. Based on the single factor experiment, the orthogonal design shows that the best conditions as follows: temperature is 75℃, the ratio of material to liquid is 1:20, the time is 2h and ultrasonic power is 200W, respectively.And under this conditions, the yield can rearch 11.03%. LBP is composed of Rha, Man, Glc and Gal. The molar ratio is 1︰1.66︰19.6︰3.98.%本文以新疆枸杞作为研究材料，采用超声波辅助法提取枸杞多糖（Lycium Barbarum Polysaccharide, LBP）。探讨了温度、料液比、时间和超声功率对多糖得率的影响，并通过高效气相对纯品LBP进行了单糖组成分析。在单因素实验的基础上进行正交实验，结果表明：温度75℃、料料比20、时间2h、超声功率160 W，得率可达11.03%。气相色谱分析单糖组成及摩尔比得LBP由鼠李糖、甘露糖、葡萄糖、半乳糖组成，摩尔比为1︰1.66︰19.6︰3.98。