目的 建立雄性大鼠肝移植术后生育模型.方法 以雄性SD大鼠作为肝移植供体和受体,供肝植入用双袖套法.共行雄性SD大鼠肝移植术15例.雄性大鼠接受肝移植术3周后,随机取5只大鼠处死行精子畸形率检测,其余大鼠与健康SD雌鼠以1︰2比例合笼饲养,随机交配产子.记录大鼠肝移植手术的一般情况;检测大鼠肝移植术后的肝功能指标;观察大鼠肝移植术后精子畸形率情况;总结雌性大鼠致孕情况和子代健康状况.结果 15只SD雄性大鼠肝移植手术成功率为100%(15/15),存活超过8周者9只(9/10).雄性大鼠肝移植术后肝功能指标均处于正常范围内,精子畸形率为0.5%~1.3%.10只肝移植术后雄性大鼠与雌鼠1︰2合笼饲养1周内,均成功使雌鼠妊娠,3周后产仔,仔鼠未见明显生理畸形.结论 本研究成功建立了雄性大鼠肝移植术后生育模型,可作为研究男性肝移植术后生育功能的动物模型.%Objective To establish male rat models for fertility following liver transplantation. Methods Male Sprague-Dawley (SD) rats were used as the donors and recipients of liver transplantation. The donor liver was transplanted with two-cuff technique. Liver transplantation was performed in 15 male SD rats. At 3 weeks after liver transplantation, 5 rats were randomly sacrificed for detection of sperm deformity rate. The remaining male rats were mixed bred and mated with healthy female SD rats at a ratio of 1︰2. General conditions of the rats undergoing liver transplantation were recorded. Liver function parameters were detected after liver transplantation. Postoperative sperm deformity rate was observed. The pregnant status of female rats and health situation of their offsprings was monitored. Results All 15 rats (100%) underwent liver transplantation successfully. Nine rats (9/10) survived longer than 8 weeks. Liver function parameters were normal in male rats following liver transplantation. The sperm deformity rate was ranged from 0.5% to 1.3%. Ten male rats undergoing liver transplantation were mixed bred with female rats at a ratio of 1︰2 for 1 week. All female rats were successfully mated and delivered their offsprings after 3 weeks. The offsprings had no evident physiological deformity. Conclusions Male rat models for fertility are successfully established after liver transplantation, which serve as an animal model to evaluate the fertility performance in male patients undergoing liver transplantation.
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