从变质的食用油中分离得到1株具有产己酸乙酯酯化酶能力的伯克霍尔德氏菌(Burkholderia sp.)X10，并对其发酵条件和酯化条件进行了优化。结果表明，该菌株最佳发酵条件为：淀粉4%，酵母膏3%，橄榄油4%，发酵液初始pH值6.5，培养温度30℃，转速180 r/min。最佳酯化条件为：己酸添加量2%，酯化温度35℃，酯化液初始pH4.6，振荡方式为连续振荡。优化条件下酯化酶活力由1.79 U/mL提高到3.53 U/mL，较优化前增加了97.21%。%A Burkholderia sp. strain X10 with esterifying enzyme-producing capability was obtained from bad cooking oil. Its fermentation and esterification conditions were optimized. The experimental results revealed its optimum fermentation conditions as follows: 4 % starch,3 %yeast extract,4%olive oil, the initial pH value of the fermenting liquid was 6.5, culture temperature was at 30℃, and the rotation speed was at 160 r/min;its optimal esterification conditions as follows:2%caproic acid,esterifying temperature was at 35℃, the initial pH value of esterify-ing liquid was 4.6, and continuous shock was adopted. Under the optimized conditions, esterifying activity got improved from 3.58 U/mL to 7.06 U/mL, 97.21%higher than that before the optimization.
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