首页> 中文期刊> 《检验医学》 >血细胞形态学室间质评方法的探索

血细胞形态学室间质评方法的探索

         

摘要

Objective To provide the high-quality external quality assessment of cytomorphology, and reduce the error caused by the differences in the course of sampling, glass slide preparation, staining and identification, in order to improve the comparability of the results of different laboratories and reflect the ability of participants fairly. Methods As the external quality assessment material of blood cell morphology, the photos taken by blood cell scanning camera were employed. Results The photos taken by blood cell scanning camera present high definition, easily identifying cellular structure. Adequate number of cells could be used to be counted. Cellavision continually scanning photos of leucocyte differentiation should be checked by manual microscopy after the analysis of software. Conclusions The blood cell photos taken by continually scanning camera can be used for cellular morphology identification and leucocyte differentiation. For external quality assessement, single cell identifying ability and leucocyte differentiating 95% and 99% confidence intervals can be used. The Clinical Laboratory Improvement Amendments in 1988 (CLIA' 88 ) analytical quality requirement as ((x) ± 3s) for examining participants' leucocyte differentiating ability also can be used.%目的 提供高质量的细胞形态学室间质评检查内容,减少因采样、制片、染色、阅片区域等过程的差异而引起的误差,提高实验事间结果 的可比性,公平、公正的体现参加者的能力水平.方法 用血片连续扫描摄像的照片作为血细胞形态室间质量评价的检查内容.结果 血片连续扫描摄像的细胞照片清晰度高,细胞结构特征明显,有足够的细胞可供计数;经连续扣描后软件分析的白细胞分类结果 须经人工复核后才能作为白细胞分类结果.结论 血片连续扫描摄像照片组成的图片文件,适用于细胞的形态识别和白细胞的分类计数.用于室间质评既可考查参加者单个细胞形态的识别能力,又可以用白细胞分类95%及99%可信限或美国临床实验室修止法案(CLIA'88)能力验证计划的分析质量要求(-x±3s)的形式来考核参加者白细胞分类的能力.

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