组蛋白乙酰转移酶p300对人乳腺癌细胞迁移的影响

摘要

To investigate the effect of p300 on tumor cell migration,p300 was overexpressed by transfecting p300 expres-sion plasmid or depleted by transfecting p300-siRNA in human breast cancer cell MDA-MB-231. Wound healing assay showed that p300 can promote the migration of MDA-MB-231 cells. RT-PCR and Western blotting analysis proved that overexpression of p300 led to the up-regulation of MYL9,CYR61 and MYH9 genes which are known to be metastasis-related. On the contrary,knockdown of p300 resulted in the down-regulation of MYL9,CYR61 and MYH9 genes.%在人乳腺癌细胞MDA-MB-231中转染p300表达质粒过表达p300，及利用siRNA干扰技术下调p300的表达，以此研究组蛋白乙酰转移酶 p300对肿瘤细胞迁移的影响．体外细胞划痕实验表明，过表达 p300可以促进 MDA-MB-231细胞的迁移．RT-PCR 和免疫印迹实验(Western blot)结果表明，过表达 p300导致肿瘤迁移相关基因 MYL9、CYR61和 MYH9的 mRNA 和蛋白表达水平的上升．与过表达 p300相反，干扰 p300的表达引起肿瘤迁移相关基因MYL9、CYR61和MYH9的mRNA和蛋白表达水平的下调．