Objective To investigate the gene expression profile of the dorsal hippocampus following transient global ischeniia in rats. Methods The rat models of glohal ischemia were established. The total RNAs of sham-operated group and ischemia group were extracted from the dorsal hippocampus after ischemia for 6 and 24 h. A1l of the RNAs were purified and converted to cDNAs by reverse transcription. The cDNAs were labeled with Cy5 (ischemia group) or Cy3 (sham-operated group) and hybridized to a rat whole-genome oligonucleotide microarray. The gene expression profiles were analyzed using bioinformatics methods. Results A total of 491 genes, including 351 genes at 6 h and 296 genes at 24 h after ishemcia , were up-regulated. A total of 387 genes, including 296 genes at 6 h and 179 genes at 24 h after ischemia , were down-regulated. And then, these differentially-expressed genes were analyzed using Cluster analysis and KEGG pathway analysis. Conclusions Hundreds of differentially-expressed genes were found by analyzing the whole-genome oligonucleotide microarray comhined with the bioinformatics approaches. This result provides some useful information for clarifying the molecular mechanism of brain ischemia injury.%目的:检测大鼠短暂全脑缺血后背侧海马组织中缺血神经细胞损伤相关差异表达基因.方法:首先建立大鼠全脑缺血动物模型,分别于缺血后6和24 h提取缺血组和假手术组的背侧海马组织RNA,将RNA逆转录合成相应cDNA,以Cy5和Cy3标记cDNA作为探针,与含有41 000点的大鼠全基因组芯片进行杂交,采用生物信息学分析基因表达谱的改变.结果:共发现上调差异基因491个,脑缺血6和24 h分别上调351和296个基因;下调差异基因387个,脑缺血6和24 h分别下调296和179个基因;并对差异表达基因进行聚类分析和信号转导通路分析等.结论:应用全基因组表达芯片并结合生物信息学软件分析大量的差异表达基因,为缺血性脑损伤的分子机制研究了提供有用的信息.
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