IL-10在H.pylori相关性胃炎组织中的表达及与COX-2、iNOS的相关性

摘要

目的:观察IL-10在H.pylori相关性胃炎组织中的表达及与COX-2、iNOS的相关性.方法:选取74例H.pylori相关性胃炎患者,均行H.pylori检测确定H.pylori感染情况,行病理组织学检测明确病理分型、胃黏膜炎症程度,行免疫组化检测胃黏膜中IL-10、COX-2、iNOS表达.分析H.pylori感染情况、病理诊断与组织分型结果,明确慢性胃炎患者H.pylori感染与病理炎症程度、病理分型的关系、IL-10表达与H.pylori感染的关系、比较H.pylori阳性胃炎不同病理类型中COX-2、iNOS表达情况,分析H.pylori阳性胃炎患者IL-10与COX-2、iNOS表达的相关性.结果:74例慢性胃炎患者中,H.pylori阳性59例,阴性15例,H.pylori检出率79.73%.H.pylori阳性与阴性患者在炎症程度的分布方面有显著差异(P＜0.05),表现为H.pylori阳性患者中重度炎症患者居多,而阴性患者则以轻度为主.74例患者中31例为IL-10阳性、43例为IL-10阴性.IL-10阳性患者中的H.pylori阳性率(93.55%)显著高于IL-10阴性(69.77%)患者(P＜0.05);病理分型的差异无统计学意义(P＞0.05).COX-2阳性表达率在H.pylori阳性患者中为59.32%,显著高于H.pylori阴性患者的20.00%(P＜0.01);iNOS阳性表达率为61.02%,显著高于阴性患者的 20.00%(P＜0.01).H.pylori阳性不同病理类型胃炎患者的IL-10阳性率差异无统计学意义(P＞0.05);H.pylori阳性胃炎(IM+Dy)患者的COX-2阳性率(84.62%)高于CSG(44.00%)患者(P＜0.05);CAG患者的iNOS阳性率(80.95%)显著高于CSG(44.00%)患者(P＜0.05).H.pylori阳性胃炎患者的IL-10表达与COX-2(r＝ -0.566,P＜0.001)、iNOS(r＝ -0.465,P＜0.001)表达均呈现显著负相关.结论:IL-10为H.pylori相关性胃炎的保护因子,在H.pylori阳性患者中具有高阳性率.且IL-10与H.pylori阳性患者的COX-2、iNOS表达呈较强负相关,可通过对COX-2、iNOS表达的下调作用降低慢性胃炎患者的恶性病变风险.%Objective:To observe the expression of IL-10 in H.pylori-associated gastritis and its correlation with COX-2 and iNOS.Methods:74 cases of H.pylori related gastritis were selected.H.pylori was tested to deter-mine H.pylori infection.The histopathological examination was tested to determine the pathological type,the degree of inflammation of the gastric mucosa.The immunohistochemistry was used to detect the expression of IL-10,COX-2 and iNOS in gastric mucosa.The H.pylori infection,pathological diagnosis and histological typing results were ana-lyzed.The relationships between H.pylori infection and pathologic inflammation and pathological type in patients with chronic gastritis were analyzed.The relationship between IL-10 expression and H.pylori infection was analyzed.The expression of COX-2 and iNOS in H.pylori-positive gastritis in different pathological types were compared.The correlations between IL-10 and COX-2,iNOS expression in H.pylori positive gastritis patients were analyzed.Re-sults:In 74 cases of chronic gastritis patients,59 cases were H.pylori positive and 15 cases were negative,the H.py-lori detection rate was 79.73%.The patients of H.pylori positive and negative was significantly different in the degree of inflammation of the distribution(P＜0.05),manifested as H.pylori positive patients with moderate to severe in-flammation,while the negative patients were mild mainly.The positive rate of H.pylori (93.55%)in IL-10 positive patients was significantly higher than that in patients with IL -10 negative (69.77%)(P＜0.05).There was no significant difference in pathological type (P＞0.05).The positive expression rate of COX-2 in H.pylori positive patients was 59.32%,which was significantly higher than that in H.pylori negative patients (20.00%)(P＜0.01),and the positive expression rate of iNOS was 61.02%,which was significantly higher than that of negative patients (20.00%)(P＜0.01).There was no significant difference in the positive rate of IL-10 between different patholog-ic gastritis H.pylori positive patients (P＞0.05).The positive rate of COX-2 (84.62%)in patients with H.pylori positive gastritis (IM+Dy)was higher than that in CSG (44.00%)patients (P＜0.05),and the positive rate of iN-OS (80.95%)in CAG patients was significantly higher than that in patients with CSG (44.00%)(P＜0.05).The expression of IL-10 in patients with H.pylori positive gastritis was significantly negatively correlated with COX-2 (r＝ -0.566,P＜0.001)and iNOS (r＝ -0.465,P＜0.001).Conclusion:IL-10 is a protective factor for H.py-lori-associated gastritis and has a high positive rate in H.pylori-positive patients.The expression of COX-2 and iNOS was negatively correlated with IL-10 in H.pylori positive patients,and could reduce the risk of malignant le-sions in patients with chronic gastritis by down-regulating the expression of COX-2 and iNOS.