Objective To investigate the correlation and expression of Interleukin-10 (IL-10) and cyclooxygenase-2 (COX-2) in Helicobacter pylori (//. Pylori) -infected gastritis, provide the clinical and experimental basis of immunothera-py of H. Pylori -infected gastritis and preventing gastric cancer. Methods Endoscopic biopsies were obtained from gastric antrum at endoscopy from 110 patients with gastritis. Three biopsy specimens were obtained from each patient. Rapid ure-ase testing, Warthin-Starry staining and HE staining were used. The samples of 74 patients fulfilled the criteria of H. Pylori scientific study. Fifty-nine patients were H. Pylori positive and 15 patients were H. Pylori negative. The expression of IL-10 and COX-2 were detected by immunohistothemistry. Results The expression of IL-10 and COX-2 in H. Pylori positive gastritis were 49. 15% and 59. 32% , which were significantly higher than those in H. Pylori negative gastritis ( 13. 33% vs 20.00% ) ( P ＜ 0.05 ). In H. Pylori positive group, the expression of IL-10 in active gastritis (32. 26% ) was significantly lower than that in inactive gastritis (67.86% ) (P ＜0. 02). The expression of COX-2 in active gastritis (74. 19% ) was significantly higher than that in inactive gastritis (42.86% ) ( P ＜0. 03). In H. Pylori positive group, the expression of IL-10 and COX-2 were 64.00% and 44.00% in chronic superficial gastritis, 38.10% and 61.90% in chronic atrophic gastritis, 38.46% and 84.62% in intestinal metaplasia and dysplasia group, respectively. This difference was not statistically significant in the expression of IL-10 (P＞0. 05). The expression of COX-2 tended to increase gradually. The expression of COX-2 was higher in intestinal metaplasia and dysplasia than that in superficial gastritis group (P ＜0.05). In H. Pylori positive group, there was a negative correlation between IL-10 and COX-2 (r = -0. 566, P ＜0.001). Conclusion The expression of IL-10 and COX-2 are associated with H. Pylori infection. IL-10 may have a protective action by inhibiting the overexpression of COX-2, which has correlation with gastritis and gastric cancer.%目的 了解IL-10、COX-2在幽门螺杆菌(H.pylori)相关性胃炎组织中的表达及相关性,为免疫治疗H.pylori相关性胃炎及预防胃癌发生提供临床和实验依据.方法 收集因上腹部不适等症状于我院行胃镜检查诊断为胃炎者110例,取胃窦部黏膜活检标本3块,分别行快速尿素酶试验、Warthin-Starry嗜银染色、HE染色.根据H.pylori科研诊断标准,110例标本中符合实验研究的共74例,其中H.pylori阳性59例,H.pylori阴性15例.免疫组化方法检测74例标本中IL-10、COX-2蛋白的表达情况.结果 IL-10、COX-2在H.pylori(+)胃炎中的阳性表达率分别为49.15％、59.32％,与H.pylori(一)组的13.33％、20.00％相比,两种因子的阳性表达率均显著增加(P＜0.05).H.pylori(+)组,IL-10、COX-2在慢性活动性胃炎中的阳性表达率分别为32.26％、74.19％,在非活动性胃炎的阳性表达率分别为67.86％、42.86％.IL-10在慢性活动性胃炎的阳性表达率低于非活动性胃炎(P＜0.02)；COX-2在慢性活动性胃炎的阳性表达率高于非活动性胃炎(P＜0.03).H.pylori(+)组,IL-10、COX-2在慢性浅表性胃炎中的阳性表达率分别为64.00％、44.00％,慢性萎缩性胃炎分别为38.10％、61.90％,肠化生及异型增生分别为38.46％、84.62％.IL-10在3组之间差异无显著性(P＞0.05)；COX-2的表达呈递增趋势,在肠化及异型增生组表达显著增高,与浅表性胃炎相比,差异显著(P＜0.05),其余各组两两相比无显著性差异(P＞0.05)；H.pylori(+)组,IL-10与COX-2的表达呈显著负相关,相关系数r=-0.566 (P ＜0.001).结论 IL-10、COX-2两种因子的产生与Hpylori感染相关；COX-2与胃癌的发生有关,是胃癌发生的早期事件,IL-10可能抑制了COX-2的过度表达及活性,在H.pylori相关胃炎及胃癌癌前病变中发挥保护作用.
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