凋亡相关基因 Bax、FasL、c - FLIP 在浆液性卵巢癌中的表达

摘要

Objective:To explore expressions of Bax,FasL,c - FLIP in serous ovarian carcinoma and their clinical significances. Methods:We examined expressions of Bax,FasL,c - FLIP in 16 serous cystadenoma,25 borderline se-rous cystadenoma,48 serous cystadenocarcinoma tissues with SP immuohistochemical method and real - time PCR. Results:Protein and mRNA expressions of Bax,FasL,c - FLIP in serous cystadenocarcinoma was significantly stron-ger than that in serous cystadenoma and borderline serous cystadenoma(P ﹤ 0. 05). Conclusion:Protein and mRNA expressions of apoptosis related gene Bax,FasL,c - FLIP in serous ovarian carcinoma are increased,which is involved in the development of ovarian cancer. This could also provide some new targets for the clinical prevention of ovarian cancer.%目的：探讨凋亡相关基因 Bax、FasL、c - FLIP 在浆液性卵巢癌中的表达及意义。方法：应用免疫组织化学 SP 法及实时定量 PCR 法检测卵巢浆液性囊腺瘤（16例）、交界性浆液性囊腺瘤（25例）、浆液性囊腺癌（48例）组织中 Bax、FasL、c - FLIP 蛋白及 mRNA 的表达情况。结果：Bax、FasL、c - FLIP 在卵巢浆液性囊腺瘤组织的细胞浆中未见明显的棕黄色颗粒沉着，而在交界性浆液性囊腺瘤、浆液性囊腺癌组织的细胞浆中可见明显的棕黄色颗粒沉着，其中以浆液性囊腺癌阳性表达最高。与卵巢浆液性囊腺瘤组相比，交界性浆液性囊腺瘤、浆液性囊腺癌中 Bax、FasL、c - FLIP mRNA 表达显著增高（P ﹤0.05）。结论：Bax、FasL、c - FLIP 凋亡相关基因在卵巢癌中蛋白及 mRNA 的表达呈一致升高趋势，可能通过调控凋亡相关途径共同参与了卵巢癌的发生发展过程，为临床上卵巢癌防治提供新靶点。