2009年昆明地区流行的肠道病毒71型基因特征分析

摘要

Objective To analyze genetic characterization of the VP1 genes of enterovirus 71 isolated in Kunming, China in 2009. Methods Six enterovirus 71 strains were isolates in Kunming, China in 2009. By using RT - PCR, the Vplgene fragments contained 891 nucleotides in all strains were sequenced. The sequences were aligned with other enterovirus 71 sequences downloaded from GenBank using Mega4. 1 software. Results Six isolated strains were closely related to other reference strains of subgroup C4. In VP1 gene, the ho-mology of nucleotide and amino acid among the six isolated strains were 95. 4% - 97. 9% and 97. 3% - 99. 7% , respectively, and 92. 6% - 99.4% and 87. 7% - 100% of homology when compared with that of strains isolated from the subgroups Cl ,C2 ,C3 and C5 , respectively. SHZH98 strain had less homology when compared to other strains of subgroup C4. The six strains had homology of 92. 6% -98.5% and 97.0% -99.7% compared to that of other subgroups on nucleotide and amino acid, respectively. Conclusion Six strains I-solated in Kunming in 2009 were belonged to subgroup C4 of enterovirus and 71. VP1 gene was more conservative.%目的 分析2009年昆明地区肠道病毒71型分离株VP1的遗传特征.方法 采用反转录-聚合酶链反应(RT PCR)法从Vero细胞分离的病毒株基因组中扩增出VP1基因,并测序；分别用Mega4.1软件分析其遗传特征.结果 中国昆明分离到的6株肠道病毒71型VP1基因的核苷酸和氨基酸同源性为95.4％～ 97.9％和97.3％～99.7％,与其他C基因型C1、C2、C3和C5的VP1的同源性为87.9％ ～89.1％和98.3％～99.1％,而与C4基因型的同源型为92.6％～98.5％和97.0％～99.7％,与SHZH98的同源性最低为92.6％和97.0％,与其他基因型的同源性为82.7％ ～84.6％和96.0％ ～97.6％.结论 2009年云南昆明流行的肠道病毒71型均为C4基因型；其VP1基因保守性较好.