Objective To investigate the expression and clinical significance of bcl-2 and NF-κB/ p65 in different subgroups of diffuse large B-cell lymphoma (DLBCL). Methods Immunohistochemical staining was used to detect the expressions of CD10, bcl-6, and MUM-1 in the DLBCL patients. According to immunohistochemical algorithm of Hans et al. DLBCL were subdivided into GCB and non-GCB/ABC subgroups and the expressions of bcl-2 and NF-κB/p65 were detected. The expressions of bcl-2 and NF-κB/ p65 in GCB DLBCL were compared with that in ABC DLBCL,and the correlation of bcl-2 and NF-κB/p65 expressions with survival in the two major subgroups of DLBCL were analyzed. Results The expression rates of bcl-2 and NF-κB/p65 proteins in DLBCL were 67.1% and 77.1%, and there was significant correlation between them. The expression rates of bcl-2 and NF-κB/p65 were 52.0 % and 56.0 % in GCB DLBCL, but were 75.6 % and 88.9 % in ABC DLBCL. The expression rates of two proteins were higher in ABC DLBCL than in GCB DLBCL. There was no significant correlation between bcl-2 and NF-κB/p65 protein expressions and overall survival within the GCB DLBCL subgroup, but bcl-2 and NF-κB/p65 expressions had a significant effect on overall survival within the ABC subgroup. Conclusion bcl-2 and NF-κB/p65 expressions are associated with poor survival in the ABC subgroup only. Hence, the significance of bcl-2 and NF-κB/p65 protein expressions should be assessed in the context of DLBCL subgroups in the future.%目的 探讨bcl-2与NF-κB/p65蛋白在弥漫性大B细胞淋巴瘤(DLBCL)不同亚型中的表达及其意义.方法 用免疫组织化学方法检测DLBCL患者CD10、bcl-6、MUM-1蛋白的表达,以Hans等的分型原则将其划分为GCB和非GCB/ABC亚型,同时标记bcl-2与NF-κ B/p65抗体,比较bcl-2与NF-κB/p65蛋白在GCB和ABC亚犁中的表达情况并分析二者与DLBCL两种主要亚型生存率的相关性.结果 bcl-2与NF-κB/p65蛋白在DLBCL中的表达率为67.1%和77.1%,二者表达呈正相关;GCB亚型中bcl-2与NF-KB/p65的表达率分别为52.0%和56.0%,ABC亚型中bcl-2与NF-K B/p65蛋白的表达率分别为75.6%和88.9%,ABC亚型中bcl-2与NF-κB/p65的表达率高于GCB亚型;在GCB亚型中,bcl-2与NF-κB/p65蛋白的表达与总生存率无显著的相关性,而在ABC亚型DLBCL中.bcl-2与NF-κB/p65蛋白的表达与生存率密切相关.结论 仅在ABC亚型DLBCL中,bcl-2与NF-κB/p65蛋白的表达是影响预后的重要不利因素,因此,需在DLBCL亚分型的基础上评估bcl-2与NF-κB/p65蛋白表达的预后意义.
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