膀胱移行细胞癌组织中NF-κB p65、uPA、Bcl-2的表达变化及其意义

摘要

Objective To observe the expression of NF-κB p65, uPA and Bcl-2 in bladder transitional cell carcinoma (here in after referred to as bladder cancer) tissue, and explore its relevance. Methods The expression of NF-κB p65, uPA and Bcl-2 in 40 cases of bladder cancer and 10 cases of normal bladder tissue were detected by immunohistochemical technique, its relationships with pathological grade, clinical stage, invasion, metastasis, recurrence and the correlation of NF-kB p65 and uPA, Bcl-2 were analized. Results The expression of NF-kB p65, uPA, Bcl-2 in bladder cancer were significantly higher than that of normal bladder tissue (P <0.05); expression of NF-kB p65 and uPA in bladder cancer has relation to pathological grade, clinical stage, lymph node metastasis (all P <0. 05), expression of Bcl-2 has relation to pathological grade, clinical stage (P<0.05), but not to lymph node metastasis(P >0.05). The expression of NF-kB p65 and uPA, Bcl-2 in bladder cancer was positively correlated( r = 0.388, 0.462, all P <0.05). Conclusions NF-κB p65, uPA and Bcl-2 in bladder cancer with high expression and have relation to clinicopathological characteristics of the bladder cancer. NF-kB p65 may promote the progress of bladder cancer by regulating uPA, Bcl-2. NF-κB p65, uPA and Bcl-2 can be used as biomarkers to decide transitional cell carcinoma of bladder invasion, metastasis and prognosis.%目的 观察膀胱移行细胞癌(下称膀胱癌)组织中NF-κB p65和尿激酶型纤溶酶原激活物(uPA)及Bcl-2的表达变化,并探讨其相关性.方法 采用免疫组化SP法对40例膀胱癌组织和10例正常膀胱组织中NF-κBp65、uPA、Bcl-2进行测定,分析三者与膀胱癌分级、分期、侵袭转移及复发的关系及NF-κB p65表达与uPA、Bcl-2的相关性.结果 NF-κB p65、uPA、Bcl-2在膀胱癌组织中的表达均明显高于正常膀胱组织(P＜0.05)；NF- κB p65和uPA与膀胱癌病理分级、分期、淋巴结转移有关(P均＜0.05),Bcl-2与膀胱癌病理分级、分期有关(P＜0.05),与淋巴结转移无关(p＞0.05)；膀胱癌组织中,NF-κB p65表达与uPA、Bcl-2表达呈正相关(r分别为0.388、0.462,P均＜0.05).结论 在膀胱癌组织中NF-κB p65、uPA、Bcl-2均呈高表达,并与膀胱癌的临床病理学特征有关；NF-κB p65可能通过调控uPA、Bcl-2的表达促进膀胱癌的进展；NF-κB p65、uPA、Bcl-2可以作为判断膀胱癌侵袭性、转移及预后的生物学标志物.