酶解法制备大豆低聚肽工艺的研究

摘要

以大豆分离蛋白为原料,采用酶解法对大豆分离蛋白进行水解制备大豆低聚肽.分别筛选出制备大豆低聚肽的最佳单酶、双酶复合.通过单因素和正交试验结果分析,确定了酶解温度55℃,水解时间2h,酶的复配比例为2:1,pH值为6.0,为最佳工艺条件.%Soy protein isolates were taking as the raw materials, this paper studied on the extraction technology of oligopeptide which were separated by enzyme, based on hydrolysis time, enzymatic hydrolysis temperature, pH value, substrate concentration, for each single factor analysis, and determined by orthogonal experiment, the results showed that the optimum technological conditions: the hydrolysis time was 2 h, the enzymatic hydrolysis temperature was 55 ℃ , pH value was 6.0, the substrate concentration was 5 g/100 mL. Using ultraviolet spectrum to compare the samples and standard products, determined that the separated materials after hydrolysis were oligopeptides. The content of soybean oligopeptide was 95.30 mg/100mL.