The best enzyme to prepare peanut peptides, papain, coupled with microwave irradiation was selected from five common proteases according to the results of the yield of peanut peptides [nitrogen solution index (NSI) in trichloroacetic acid (TCA), TCA-NSI] and the degree of hydrolysis (DH). The main factors that influenced the microwave-coupled enzymatic digestion method were optimized by response surface analysis. The optimal conditions obtained were as follows: microwave extraction time, 9.5 min; power, 600 W; substrate concentration, 4%; enzymatic reaction temperature, 50 °C; enzyme quantity, 6,500 U/g; pH, 7.1 (phosphate buffer, 0.05 mol/L). Under these conditions, TCA-NSI was 62.00% and DH was 25.89%, which is higher than that obtained with either protease hydrolysis or microwave hydrolysis alone.
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机译：根据花生肽[三氯乙酸（TCA），TCA-NSI中的氮溶液指数（NSI）和水解度（DH）。通过响应面分析优化了影响微波耦合酶消化方法的主要因素。获得的最佳条件如下：微波提取时间为9.5分钟;功率600 W;底物浓度为4％;酶促反应温度为50°C;酶量6,500 U / g; pH值7.1（磷酸盐缓冲液，0.05 mol / L）。在这些条件下，TCA-NSI为62.00％，DH为25.89％，高于单独使用蛋白酶水解或微波水解获得的TCA-NSI。
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