驴肉及其制品真伪鉴别技术研究进展

摘要

Donkey meat and its products are very popular with high nutritional value and unique taste.Due to the high price of donkey meat,the market appeared adulteration sales,which greatly violated the legitimate rights and interests of consumers,even to the health of people,and disrupted the market integrity.However,China has not yet established quality supervision and inspection standards for donkey meat products at present.Therefore,there is an urgent need to establish simple,fast and accurate methods for detecting donkey meat products.In recent years,polymerase chain reaction (PCR) and its derivative technology have become a hot topic in the identification of the authenticity of donkey meat products.Emerging DNA barcodes and digital PCR have great application potentiality.This paper summarized the research progress of detection methods of donkey meat and its products with morphology,metabolites,proteins and nucleic acids,which mainly included spectroscopy,chromatography,enzyme-linked immunosorbent assay,PCR and its derivative technologies.%驴肉及其制品因具有极高的营养价值和独特的味道而深受人们喜爱.因其价格居高不下,市场上存在商贩掺假销售问题,极大侵犯了消费者的合法权益甚至危害身体健康,且扰乱了市场诚信.目前,中国尚未建立驴肉产品质量监督检测标准.因此,迫切需要建立一种简单、快速、准确的驴肉产品检测方法.近几年来,聚合酶链反应(polymerase chain reaction,PCR)及其衍生技术成为鉴别驴制品真伪的研究热点,新兴的DNA条形码和数字PCR技术也具有较大的应用潜力.本文综述了驴肉及其制品的形态、代谢物、蛋白质、核酸检测方法的研究进展,这些方法主要包括光谱法、色谱法、酶联免疫吸附法、PCR及其衍生技术等.