四磨汤对慢性应激小鼠胃肠运动及脑内NT、CGRP的影响

摘要

目的 通过研究四磨汤对慢性应激小鼠胃肠运动功能和脑内神经降压素(NT)、降钙素基因相关肽(CGRP)的影响,进一步探讨四磨汤治疗功能性胃肠病的作用机制.方法 采用饥饱失常、明暗颠倒以及束缚夹尾等多种方法 对小鼠造模,分别给予蒸馏水、吗丁啉、四磨汤治疗,观察胃排空和小肠推进功能,免疫组织化学法观察脑组织NT、CGRP的表达.结果 模型组小鼠胃排空和小肠推进功能低于正常组,脑组织中NT、CGRP含量高于正常组;四磨汤组胃肠运动功能恢复正常,脑组织中NT、CGRP含量低于模型组,与正常组比较差异无统计学意义.结论 四磨汤调节脑内NT、CGRP的表达,可能是其治疗功能性胃肠病的作用机制之一.%Objective:To study the effect of Simo Decoction on gastrointestinal motility,neurotensin (NT) and calaitonin gene related peptide (CGPR) in brain tissue of mice with chronic stress and to explore the mechanism of Simo Decoction on functional gastrointestinal disorders (FGIDs). Methods :The mice models were established by exerting the factors of irregular diet, reverse of day and night, binding and stimulating tails. And then they were treated with distilled water,domperidone and Simo Decoction. The function of gastric emptying and the small intestinal propulsion were observed. Expressions of NT and CGRP were measured by immunohistochemical staining.Results:The function of gastric emptying and the small intestinal propulsion of the model group were lower siguificantly than those of the normal group. Expressions of NT and CGRP in brain tissue of the model group were higher obviously than those of the normal group. In the Simo Decoction group,the NT level and the CGRP concentration were lower than those in the model group. However,there was no statistical difference between this group and the normal group in the NT content and the CGRP level. Conclusion:One of the mechanisms of treating FGIDs is that Simo Decoction can regulate expressions of the NT and CGRP.