急性应激对小鼠空间学习记忆功能及脑内NT-3表达的影响

摘要

Objective: To study the effects of acute stress on the spatial learning-memory function of mice and its mechanism. Methods: Using acute stress model mice induced by foot shock. The ability of spatial learning-memory of mice were determined by Morris Water maze task, and the expression of nerve neurotrophic factor 3 (NT-3) in the hippocampus (HP) and prefrontal cortex (PFC) were detected by immunohistochemical method. Results:The ability of spatial learning and memory in stress group mice were significantly enhanced (P<0.01), compared with the control group mice. The NT-3 expression in CA1,CA3, DG of HP and PFC were significantly increased in stress group mice (P<0.05, P<0.01). Conclusion: The acute stress causes the improvement of spatial and memory function in mice which may be closely related to the up-regulation of NT-3 expression in brain.%目的：研究急性应激对小鼠空间学习记忆的影响及其机制。方法：以足底电击来建立急性应激小鼠模型，采用Morris水迷宫检测小鼠空间学习记忆能力，以免疫组化方法检测神经营养因子-3(NT-3)在小鼠海马和前脑皮层的表达。结果：水迷宫实验中，急性应激组小鼠与对照组相比，空间学习记忆显著提高(P<0.01)。在海马CA1区、CA3区、齿状回及前脑皮层NT-3的表达显著增强(P<0.05，P<0.01)。结论：急性应激所致小鼠空间学习记忆功能的提高可能与脑内NT-3表达上调相关。