乙型肝炎不同临床类型患者血清维生素C及氧化损伤指标的检测

摘要

目的 对比检测乙型肝炎不同临床类型患者血清维生素C含量及其它氧化损伤指标,为维生素C在乙型肝炎患者的临床应用提供参考依据.方法 选取乙型肝炎患者共45例[慢性乙型肝炎(重度)20例、肝炎肝硬化<代偿期)15例、重型肝炎10例],健康对照组15例,采用紫外分光光度仪测定血清维生素C、总抗氧化能力及丙二醛,并进行对比分析.结果 (1)与健康对照组比较,乙型肝炎组血清维生素C、总抗氧化能力明显降低(P<0.05);不同临床类型间均存在显著差异:重型肝炎组<慢性肝炎组<肝硬化组<正常组(P<0.05);乙型肝炎组丙二醛较健康对照组明显升高(P<0.05):重型肝炎组>慢性肝炎组>肝硬化组>正常组(P<0.05).(2)乙型肝炎组血清维生素C的含量与总抗氧化能力呈正相关,与丙二醛水平呈负相关.结论 乙型肝炎患者血清维生素C水平及总抗氧化能力较健康对照组明显降低,丙二醛含量明显升高,维生素C的浓度与与总抗氧化能力呈正相关,与丙二醛含量呈负相关.%Objective To detect vitamin C levels and other indicators of oxidative damage in serum of patients with different clinical types of hepatitis B, and toprovide reference for use of Vitamin C in hepatitis B patients.Methods 45 cases of hepatitis B patients of which 20 patients were severe chronic hepatitis B patients, 15 cases were of compensated liver cirrhosis, 10 cases were of severe hepatitis,and 15 healthy patients as control group were included in the study.Vitamin C, malondialdehyde and total antioxidation were measured by ultraviolet spectrophotometry.Results (1)Compared with the healthy control group, vitamin C and total antioxidant capacity in hepatitis B patients’ serum were significantly decreased (P＜0.05).Different clinical types have significant differences: severe hepatitis group ＜chronic hepatitis ＜ cirrhosis group ＜ normal group (P＜0.05).Hepatitis B groups’ malondialdehyde level was significantly higher than that in the healthy control group (P＜0.05): severe hepatitis group ＞ chronic hepatitis ＞ liver sclerosis group ＞ control group (P＜0.05).(2)The concentration of vitamin C in hepatitis B patients’ serum was positively correlated with total antioxidant capacity and was negatively correlated with MDA.Conclusion The vitamin C level and total antioxidant capacity in hepatitis B patients’ serum were significantly lower than that in the healthy control group, and MDA content was significantly increased.The concentration of vitamin C was positively correlated with total antioxidant capacity and was negatively correlated with MDA.