In order to explore the application of ultrasonic and microwave technology in ACE inhibitory peptide preparation of sesame cake,using ultrasonic and microwave on sesame cake pretreatment respectively,ultrasonic as-sisted enzymatic hydrolysis,investigating the effects of ultrasonic power,ultrasonic time,microwave power,micro-wave time,enzyme dosage inhibition rate of ACE.The results show that:the ultrasonic pretreatment power is 4 W/mL,with pretreatment of 10 min,pretreatment with 1 300 U /g alkaline protease of sesame cake ACE IC50 value is 2.81 mg/mL.Ultrasonic assisted enzymatic hydrolysis process of ultrasonic power is 0.5 W/mL,add 1 700 U /g alkaline protease,enzyme solution is obtained at 15 min,sesame cake ACE inhibitory peptide of IC50 value is 2.96 mg/mL.Microwave power is 1.33 W/mL,microwave pretreatment at 5 min inhibitory peptides from sesame cake ACE IC50 value is 2.81 mg/mL.%为了探索超声波和微波技术在芝麻饼粕 ACE 抑制肽制备中的应用,分别以超声波和微波对芝麻饼粕预处理,超声波辅助酶解,研究了超声波功率、超声波时间、微波功率、微波时间和加酶量对 ACE 抑制率的影响。结果表明:超声波预处理功率为4 W/mL、预处理10 min、添加1300 U /g 碱性蛋白酶时得到的芝麻饼粕 ACE 抑制肽的 IC50值为2.81 mg/mL。超声波辅助酶解过程中超声功率选择0.5 W/mL、添加1700 U /g碱性蛋白酶、酶解15 min 时得到的芝麻饼粕 ACE 抑制肽的 IC50值为2.96 mg/mL。微波预处理功率为1.33 W/mL、微波预处理5 min 时得到的芝麻饼粕 ACE 抑制肽的 IC50值为2.81 mg/mL。
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