4种培养基对红曲霉M1莫纳可林K产量影响及基因差异表达分析

摘要

Monacolin K is one of the important secondary metabolites of Monascus sp. The influence of Monacolin K production for Monascus M1 on four kinds of fermentation substrate were studied. And the mRNA expression of Monacolin K biosynthesis gene cluster was measured by RT-qPCR. The result showed that the concentration of Monacolin K was the highest(8. 49 mg/L) cultured by buckwheat while the concentration of Monacolin K was 7. 12, 4. 77, 2. 11 mg/L cultured by brown rice, oats, and rice. And there was positive correlation between the expression genes related to MK and the MK production, except for MKB and MKC. The different kinds of culture medium could influence the secondary metabo-lites production by stimulating the gene clusters.%莫纳可林K是红曲霉重要的次级代谢产物之一。研究了4种培养基质对红曲霉M1莫纳可林K产量的影响，并利用RT-qPCR的方法，对莫纳可林K相关基因簇mRNA水平的差异表达进行测定分析。结果表明，荞麦培养基中莫纳可林K的产量最高(8.49 mg/L )，其次为糙米(莫纳可林K产量7.12 mg/L)、燕麦(莫纳可林K产量4.77 mg/L)、大米(莫纳可林K产量2.11 mg/L)。荞麦培养基利于红曲霉莫纳可林K产生。基因差异表达研究发现，除MKB与MKC基因外，其他基因的表达量均与莫纳可林K产量呈正相关；不同培养基通过刺激红曲霉基因表达从而影响其次级代谢产物产生。