高产碱性果胶酶基因工程菌的发酵条件的优化

摘要

In order to in prove the alkaline pectate lyase (PEL ) production in pW B 980- pel521/W B600 , three key factors such as the concentration of bean cake powder , phosphate and NaCl were selected by P lackett-Burm an method through single factor experin ent in shake flask . Mean while , response surface analysis was used to optinize these factors . The results showed that the optim almedium contained wheat bran 30 .00 g/L , bean cake powder 35 .3345 g/L , phosphate 0.04552 mol/L , N aCl4 .2988 g/L , C aCl2 1.11 g/L . Under the optrnized conditions , the alkaline pectate lyase activity was in proved to 760 U/mL .%为了提高碱性果胶酶基因工程菌(pWB980-pel521/WB600)产酶能力,实验室在摇瓶条件下采用Plackett-Burman (P-B) 方法筛选出对产酶有重要影响的3个因素(豆饼粉、磷酸盐以及氯化钠的添加量),并采用响应面试验设计(RSM) 对重要因素进行优化.结果表明,摇瓶发酵培养基成分为:麸皮30.00 g/L(浸汁)、豆饼粉35.3345 g/L、磷酸盐0.04552 mol/L、氯化钠 4.2988 g/L、氯化钙1.11 g/L的条件下,碱性果胶酶基因工程菌产酶能力得到了显著提高,酶活力达到约760 U/mL,产酶能力比发酵优化前提高了3倍.