ERα、ERβ和EGFR在人肺癌组织芯片中的表达和意义

摘要

目的 探讨雌激素受体α(ERα)和β(ERβ)及表皮生长因子受体(EGFR)在肺癌患者肿瘤组织中的表达及意义.方法 选取癌前病变组(不典型腺瘤样增生)12例,原发肺癌组77例,淋巴结转移性肺癌组12例构建成进展组织芯片.应用免疫组化方法检测ERα、ERβ和EGFR在此芯片中的表达,并探讨其相互关系.结果 在进展组织芯片癌前病变组、原发肺癌组及淋巴结转移性肺癌组中ERα的表达阳性率分别为91.67%、62.34%和25.00%,不同组间比较差异有统计学意义(P<0.01);ERβ的表达阳性率分别为91.67%、55.84%和16.67%,不同组间比较差异有统计学意义(P<0.01);EGFR的表达阳性率分别为25.00%、59.74%和91.67%,不同组间比较差异有统计学意义(P<0.01).在原发肺癌中,ERα表达与性别、组织学分型、肿瘤分化程度及P-TNM分期有关(P<0.05),ERβ表达与性别、组织学分型、肿瘤分化程度、P-TNM分期及淋巴结转移有关(P<0.05),EGFR表达与淋巴结转移及肿瘤的P-TNM分期有关(P<0.05).ERβ和EGFR的表达呈正相关(r=0.612,P<0.01).结论 ERα、ERβ及EGFR均与肺癌的发生发展有关,且ERβ和EGFR的表达相关,这种潜在的机制及关联性为将来的进一步研究提供了基础.%Objective To investigate the expression and biological significances of estrogen receptorα(ERα), estrogen receptorβ(ERβ), and epidermal growth factor receptor (EGFR) in lung cancer. Methods We constructed a tis-sue microarray in which there were 12 cases of precancerous lesion, 77 cases of primary lung cancer, and 12 cases of lung cancer with lymph node metastasis, and detected the expression of ERα, ERβand EGFR in these specimens by us-ing Elivision immunohistochemical method. Results The positive rate of ERα was 91.67% in precancerous tissues, 62.34%in primary lung cancer and 25.00%in lung cancer with lymph node metastasis, and there were significant differ-ences among them (P<0.01). The positive rate of ERβwas 91.67%in precancerous tissues, 55.84%in primary lung can-cer and 16.67% in lung cancer with lymph node metastasis, and the differences were statistically significant (P<0.01). The positive rate of EGFR was 25.00%, 59.74%, and 91.67%, respectively, and the differences among them were signifi-cant (P<0.01). In primary lung cancer, the expression of ERα was significantly correlated with gender, histological types, grade and P-TNM stage (P<0.05), and the expression of ERβwas significantly correlated with gender, histological types, grade, P-TNM stage and lymph node metastasis (P<0.05). The expression of EGFR was significantly correlated with lymph node metastasis and P-TNM stage (P<0.05). Correlation analysis showed that there was a positive correla-tion between ERβand EGFR (r=0.612, P<0.01). Conclusion The expression of ERα, ERβand EGFR are related to the occurrence and development of lung cancer, and the expression of ERβis correlated with EGFR. The underlying mecha-nism and potential translational relevance provide the foundation for further investigation.