不同溶剂绞股蓝提取物清除自由基性能

摘要

分别以80℃水和25℃50%的乙醇溶液为提取溶剂,固液比1：30,以普通震荡法提取绞股蓝中黄酮类(包括儿茶素、原花青素)物质,紫外显色法测定两种提取液中黄酮类物质含量。结果表明：提取液中黄酮类物质含量分别为18.0 mg/g和6.00 mg/g,提取液温度对绞股蓝中黄酮类物质的提取效果影响较大。80℃水提取液比25 ℃50%的乙醇提取液清除DPPH自由基的能力更强；清除自由基能力与提取物中黄酮类物质含量并不完全呈正比。%To optimize the extracting total flavonoids, 80 ℃ water and 25 ℃ 50% ethanol were used to extract the flavanolin Gynostemma, include catechin and polyprocyanidin. The contents of flavanols were determined in two extracts. The contents of flavanol were 18. 0 mg/g by 80℃ water and 6. 00 mg/g by 50% ethanol. The 80℃ water was batter than 50% ethanol on extracting the contents of flavanol in Gynostemma. The 80 ℃ water extracts were more batter on scavenging of DPPH (1,1-Diphenyl-2-picrylhydrazyl) than 50% ethanol. It was no effective on free radical scavenging activity to contents of flavanols.