Preparation method of Gynostemma pentaphyllum leaves extract and Gynostemma pentaphyllum extract prepared by the method thereof

摘要

A preparation method of Gynostemma Pentaphyllus leaves extracts with increased amount of small molecular effective saponin and decreased benzopyrene includes the steps of drying fresh Gynostemma Pentaphyllus leaves after heating, treating the dried Gynostemma Pentaphyllus leaves with steam, adding water of 1 to 100-fold volume to the above Gynostemma Pentaphyllus leaves and then preparing a hot water extract by carrying out a hot water extraction at 100-180 V, 0.5-10 atm for 1-120 hours, adding C₁-C₄ lower alcohol of 1 to 100-fold volume to the residual remained after the hot water extraction and then preparing an alcohol extract of the residual from the hot water extract by carrying out an alcohol extraction at 50-100° C. for 1-4 hours, and mixing the hot water extract and the alcohol extract, and then filtering and concentrating the mixture.