苦瓜花粉母细胞减数分裂行为研究

摘要

研究苦瓜花粉母细胞的减数分裂行为,发现苦瓜花粉母细胞减数分裂中细胞质分裂类型为同时型,并且在减数分裂过程中存在不同步现象.在苦瓜花粉母细胞细胞核分裂中,从前期Ⅰ到中期Ⅰ,核仁逐渐消失,到前期Ⅱ又重新出现,随后消失.同时,从前期Ⅰ到中期Ⅰ,染色体开始收缩并逐渐变得清晰,到末期Ⅰ时,苦瓜染色体发生解螺旋并逐渐模糊,前期Ⅱ再次变得清晰.研究还发现,苦瓜花粉母细胞减数分裂前期Ⅰ和前期Ⅱ中,存在多核仁现象.苦瓜减数分裂过程中产生部分多分孢子,这可能与逆境及染色体不同步分离相关.%Meiotic behaviors of pollen mother cells (PMCs) of bitter gourd (Momordica charantia L. ) were characterized. It was found that the pollen mother cell of bitter gourd revealed a simultaneous cytokinesis with unsynchronized phenomenon in meiotic division. Nucleolus disappeared gradually from prophase I to metaphase I, reappeared at prophase II and then disappeared. Chromosome became more and more identifiable from prophase I to metaphase I, and illegible at telophase I with unwinding. Moreover, multi nucleoli was investigated in prophase I and prophase II. Frequent polyads were observed in this study, which was likely related to the weather adversity and unsynchronized phenomenon in meiotic division.