The separation performance of columns with different polarities,including HP-5,DB-Wax and SPB-50 column,for 37 fatty acid methy esters(FAMEs) was compared by gas chromatography-mass spectrometry(GC-MS).The results showed that these columns allowed FAMEs separation.The peaks of FAMEs with less carbon atoms were separated earlier.FAMEs with the same number of carbon atoms and different degree of saturation were separated in different orders on the stationary phases.SPB-50 column could separate cis/trans,ω-3 and ω-6 fatty acids more sufficiently and may be a more suitable column for complicated FAME sample analysis,especially for functional fatty acids.%利用气相色谱-质谱联用技术,考察HP-5、DB-Wax和SPB-50三种不同极性色谱柱对37种脂肪酸甲酯(fattyacid methy ester,FAME)混标的分离性能,并对实际鱼油样品的脂肪酸进行检测。结果表明:3种色谱柱都能用于FAME的分析,低碳数的物质最先出峰,同碳数而饱和度不同的FAME在不同固定相上的出峰顺序不同;与其他两种色谱柱相比,SPB-50柱能分离出顺式和反式异构体以及有效分离ω-3与ω-6系列脂肪酸,更适合检测复杂的FAME混合物样品,特别是对顺式和反式脂肪酸以及ω-3(如EPA和DHA)和ω-6等功能性脂肪酸的检测有重要意义。
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