水溶性大豆膳食纤维对高脂血症小鼠肾脏的抗氧化应激作用

摘要

目的：观察不同工艺制备的大豆膳食纤维对高脂血症小鼠肾脏抗氧化应激的作用。方法：采用超声辅助提取法和碱提醇沉法分别提取大豆膳食纤维,用高脂饲料制造高脂血小鼠模型,两种工艺制备的大豆膳食纤维分别分为高（400mg/（kg.d））、中（200mg/（kg.d））、低（100mg/（kg.d））3个剂量组,连续灌胃15d,检测小鼠肾组织中相关的生化指标。结果：大豆膳食纤维能够降低高脂血小鼠肾脏中谷胱甘肽-S转移酶（GST）、H2O2、丙二醛（MDA）含量,提高其谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）酶活力。结论：两种工艺制备的大豆膳食纤维提高了小鼠肾的抗氧化应激效应,超声辅助提取法制备的大豆膳食纤维灌胃的小鼠肾组织抗氧化效应略优于碱醇沉提法组,并与剂量相关。%Objective： To explore the anti-oxidative stress effect of water-soluble soybean dietary fiber（WSSDF） prepared by ultrasound-assisted extraction（UAE） or alkali extraction（AE） on the kidneys of hyperdipemic mice.Methods： Kuming male mice were randomly divided into 8 groups.The mouse groups were fed a normal diet or a high fat diet alone or supplemented with WSSDF（400,200mg/（kg·d） and 100 mg/（kg·d）） derived from UAE and WSSDF（400,200 mg/（kg·d） and 100 mg/（kg·d）） derived from AE,respectively.After 15 consecutive days of intrasgastric SDF administration,serum TC,TG,HDL-C and LDL-C and kidney MDA,H2O2,GSH,GST,SOD and CAT were determined.Results： Compared with the model group,both WSSDFs derived from UAE and AE could decrease the contents of GST,MDA and H2O2,increase the activities of GSH,SOD and CAT in the kidneys of hyperdipemic mice.Conclusion： WSSDF can improve antioxidant stress in kidney of hyperdipemic mice in a dose-dependent manner and UAE results in a slight increase of the activity of WSSDF when compared with AE.