HPLC法测定不同产地黑豆皮中矢车菊素-3-O-葡萄糖苷的含量

摘要

Objective： To determine cyanidin-3-O-glucoside（C3G） content in black soybean hulls grown in different regions of China by HPLC.Methods： The chromatographic separation was performed on Phenomenex Luna Su C18（250 mm × 4.60 mm,5 μm） column by gradient elution using 0.5% phosphoric acid solution as mobile phase A and water： acetonitrile（50：50,V/V） as mobile phase B at a flow rate of 0.8 mL/min.The column temperature was set at 30 ℃and the detection wavelength at 520 nm.Results： The developed regression equation of cyanidin-3-O-glucoside standard is： Y = 2 × 107X-33120（r= 0.9998）.A good linearity was shown in the range of 0.1041-1.041 μg.The average recoveries at three spike levels were 92.4%,92.5% and 95.5%,respectively.The contents of cyanidin-3-O-glucoside in samples from Northeast China and Shandong,Hubei and Anhui provinces were in the range of 5.263-12.829 mg/g.Conclusions： The method is simple and reliable,and may be used for the quality control of black soybean hull from different geographic origins.%目的：建立黑豆皮中矢车菊素-3-O-葡萄糖苷的含量测定方法,测定5个不同产地黑豆皮中矢车菊素-3-O-葡萄糖苷的含量。方法：采用HPLC法测定黑豆皮中矢车菊素-3-O-葡萄糖苷的含量,色谱柱采用Phenomenex LunaSu C18柱（250mm×4.60mm,5μm）;流动相A相为0.5%磷酸溶液,B相为水-乙腈（50：50）,进行梯度洗脱;流速0.8mL/min;检测波长520nm;柱温30℃;进样量10μL。结果：矢车菊素-3-O-葡萄糖苷标准曲线回归方程为：Y=2×107X-33120（r=0.9998）,在0.1041～1.041μg范围内线性关系良好,平均回收率分别为92.4%、92.5%和95.5%,不同产地黑豆皮中矢车菊素-3-O-葡萄糖苷含量范围为5.263～12.829mg/g。结论：所用方法简便、准确,可用于不同产地黑豆皮的质量控制。