RP-HPLC法测定青娥丸提取物中松脂醇二葡萄糖苷含量

摘要

目的建立测定青娥丸提取物中松脂醇二葡萄糖苷含量的RP-HPLC法.方法青娥丸提取物用75%乙醇提取,经AB-8大孔吸附树脂纯化后,注入高效液相色谱仪.本法采用0DS分析柱为固定相,以甲醇-乙腈-水(24:3:78,V/V/V)为流动相,流速为1.0 mL·min-1,紫外检测波长为227nm.结果松脂醇二葡萄糖苷的线性范围为5.5-170μg·mL-1(r＞0.9998),平均回收率99.3%.日内和日间精密度分别为1.3%和2.8%(n=5).青娥丸中松脂醇二葡萄糖苷的含量为0.446±0.012mg·g-1(n=10).结论本方法灵敏、准确,专属性强,适用于青娥丸及其他复方中药中松脂醇二葡萄糖苷的含量测定.%Aim To develop and determine pinoresinol diglucopyranoside in Qing'e Pill, a traditional Chinese com-pound preparation containing Eucommia ulmoides Oliv. as the principal drug, by a reverse-phase high-performance liquid chromatographic method (RP-HPLC). Methods The extract of Qing'e Pill was refiuxed with 75% ethanol, purified on an AB-8 macroporous adsorption resin column and then injected into HPLC system. The HPLC assay was performed on an ODS analytical column with a mixture of methanol-acetonitrile-water (24:3:78, V/V/V) as the mobile phase at a flow-rate of 1.0 mL· min-1, and a UV detector set at 227 nm. Results Good linearity between peak area and concentration was found in the range of 5.5- 170 μg·mL-1 for pinoresinol diglucopyranoside ( r ＞ 0.9998). The average recovery was 99.3%. The intra-day assay RSD and the inter-day assay RSD were 1.3% and 2.8%, respectively ( n = 5). The content of pinoresinol diglucopyranoside in Qing'ePillwasdetermined to be 0.446±0.012mg·g-1(n= 10). Conclusion TheRP-HPLC meth-od was proved to be sensitive, specific, accurate and precise for the determination of pinoresinol diglucopyranoside in Qing'e Pill.