HPLC法测定不同品种苹果中原花青素B2的含量

摘要

目的：建立苹果中原花青素B2含量的测定方法，测定5个不同品种苹果果皮和果肉中原花青素B2的含量。方法：采用HPLC法测定苹果果皮和果肉中原花青素B2的含量，色谱柱为PhenomenexLunaC18柱(250mm×4.6mm，5μm)；流动相：A相：0.5%磷酸溶液，B相：水-乙腈(50：50，V/V)；流速：1.0mL/min；柱温：30℃；检测波长：280nm。结果：原花青素B2进样量在2.399～9.596μg范围内与峰面积线性关系良好(R2＝0.9998)，回收率97.72%， RSD=1.98%。经测定几种苹果果皮和果肉中原花青素B2的含量分别在275.24～548.42μg/g和90.19～247.06μg/g范围中。结论：本法简便、易行，可用于苹果质量控制。%Objective: To determine the proanthocyanidin B2 content of five apple cultivars by HPLC. Methods: The chromatographic separation was performed on a Phenomenex Luna C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase comprised water containing 0.5% H3PO4 (A) and acetonitrile-water (50:50, V/V, B) at a flow rate of 1.0 mL/min. The column temperature was 30 ℃. The detection wavelength was set as 280 nm. Results: There was a good linear relationship between peak area and concentration over the range of 2.399–9.596 μg (R2 = 0.9998). The average spike recovery of proanthocyanidin B2 was 97.72% with a RSD of 1.98%. The procyanidins B2 contents of the peel and pulp of several apple varieties were 275.24–548.42 μg/g and 90.19–247.06 μg/g, respectively. Conclusion: This method is simple, fast and reproducible and can be used for the determination of proanthocyanidin B2 in apple.