响应面法优化产赤藓糖醇菌株RH-UV-L4-F9的发酵条件

摘要

为进一步提高糖蜜玉米浆发酵生产赤藓糖醇的产量,以高产赤藓糖醇耐高渗酵母RH-UV-L4-F9为出发菌株,采用响应面法优化发酵工艺参数。根据Box-Benhnken设计原理,在单因素试验基础上,采用三因素三水平响应面分析,确定最佳发酵工艺参数为：温度32.3℃、pH5.1、摇床转速176r/min。在此优化条件下赤藓糖醇产量达到166.89mg/mL,是初始条件下的1.2倍。%This work reports the application of response surface methodology based on a three-variable,three-level BoxBenhnken design to optimize fermentation parameters to enhance the production of erythritol from molasses and corn syrup using yeast RH-UV-L4-F9,a potent erythritol producing strain.The optimal values of temperature,pH and shaking speed were 32.3 ℃,5.1 and 176 r/min,respectively.Under the optimized conditions,the yield of erythritol was 166.89 mg/mL,which was 1.2-fold higher than before the optimization.