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Recombinant Escherichia Coli and Application Thereof in Screening Erythritol-producing Strains

机译:重组大肠杆菌及其在产赤藓糖醇菌株的筛选中的应用

摘要

The present disclosure discloses recombinant Escherichia coli and application thereof in screening erythritol-producing strains, and belongs to the technical field of microorganisms. The recombinant Escherichia coli used in a method for screening an erythritol-producing strain disclosed by the present disclosure can well perform positive correlation induction on erythritol with different concentrations, so that the method for screening the erythritol-producing strain has the advantage of high sensitivity. High-concentration glucose is usually adopted as a fermentation substrate when erythritol is produced in a fermentation mode in the industry, but the method for screening the erythritol-producing strain disclosed by the present disclosure can overcome the interference of the high-concentration glucose, and under the interference of the high-concentration glucose, the recombinant Escherichia coli used in the method for screening the erythritol-producing strain can still well perform positive correlation induction on erythritol with different concentrations, and the correlation is higher than that without the interference of the glucose. Therefore, the method for screening the erythritol-producing strain has the advantage of strong anti-interference capability.
机译:本发明公开了重组大肠杆菌及其在产赤藓醇生产菌的筛选中的应用,属于微生物技术领域。本发明公开的用于筛选产生赤藓糖醇的菌株的方法中的重组大肠杆菌可以很好地对不同浓度的赤藓糖醇进行正相关诱导,因此筛选所述产生赤藓糖醇的菌株的方法具有灵敏度高的优点。在工业上以发酵模式生产赤藓糖醇时,通常采用高浓度葡萄糖作为发酵底物,但是本发明公开的筛选赤藓糖醇产生菌株的方法可以克服高浓度葡萄糖的干扰,并且在高浓度葡萄糖的干扰下,用于筛选产生赤藓糖醇菌株的方法中的重组大肠杆菌可以很好地对不同浓度的赤藓糖醇进行正相关诱导,且相关性更高比没有葡萄糖的干扰。因此,筛选产生赤藓糖醇的菌株的方法具有抗干扰能力强的优点。

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