以安徽怀远所产的“玉石籽”石榴为试材，研究乙醇体积分数、提取温度、提取时间、螯合剂六偏磷酸钠的添加量、pH值及料液比对提取效果的影响，并对提取工艺进行优化；分别提取石榴籽种壳及种仁中的多酚类物质，比较石榴籽种壳及种仁提取物中总酚、黄酮及原花色素含量及体外抗氧化能力。结果表明：在pH4．0、提取温度60℃、六偏磷酸钠添加量0．4％、液料比20：1的条件下，用60％乙醇提取100min，石榴籽中多酚的提取效果较好；石榴籽富含原花色素，原花色素是石榴籽抗氧化成分中的主要成分，石榴籽中多酚类物质-尤其是原花色素主要集中于种仁部位。%Orthogonal array design was employed to optimize the extraction of polyphenols from the seeds of pomegranate cultivar＂Yushizi＂ grown in Huaiyuan of Anhui province. The extraction efficiency of polyphenols was investigated with respect to ethanol concentration, temperature, extraction time, addition level of sodium hexametaphosphate as a chelating agent, pH and material/liquid ratio. The total phenol, total flavonoid, total proanthocyanidin contents of the whole seeds, kernels and husks of pomegranate and the in vitro antioxidant effects of their polyphenol extracts were compared. Better results for the extraction of polyphenols were achieved after 100 min of extraction under the conditions： pH 4.0, 60℃, 0.4% sodium hexametaphosphate addition, and 20：1 solid-to-liquid ratio. Pomegranate seeds were rich in proanthocyanidins as the major antioxidant components in it. Polyphenols especially proanthocyanidins were mainly present in the seed kernels of pomegranate.
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