DiversiLab系统沙门氏菌分子分型评价

摘要

目的：评价DiversiLab（DVL）系统对沙门氏菌（SA）的分型能力。方法：用rep-PCR技术为原理的DVL系统对进出口食品中分离的44株SA进行分子分型,并与脉冲场凝胶电泳（PFGE）结果比较,探讨DVL对SA的种群分类能力和分辨率。结果：44株SA经rep-PCR分型分成22个群,分离自不同国家、不同食品中的相同血清型SA分布在同一个群,而且菌株之间相似性非常高。在rep-PCR结果中分在同一个群中的SA,在PFGE结果中除SA2和SA15外,其他菌株之间相关性较低。结论：DVL在SA的种群的分类能力上优于PFGE,但分辨率低于PFGE。%Objective： To evaluate the typing performance of DiversiLab system（DVL） for Salmonella.Method： A DVL system,which is based on rep-PCR,was used to type 44 Salmonella isolates from exported and imported foods and compared with pulsed-field gel electrophoresis（PFGE）.The Salmonella isolates were typed by rep-PCR into 22 groups.The same serotypes of Salmonella isolates from different countries and foods were distributed in the same group with a high similarity.The PFGE results obtained for Salmonella isolates assigned to the same group using rep-PCR indicated a low similarity among all other isolates except No.2 and No.15.Conclusion： DVL shows better typing ability but lower resolution for Salmonella than PFGE.